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文献和实验AMNIOTIC FLUID CULTURES ON COVERSLIPS
: FISHER cat #A412-500. Methyl alcohol anhydrous AR (ACS)(Absolute) Acetone free, 500 ml bottle. Nutrient Mix F-10: GIBCO cat# 81200-040. Nutrient Mixture F-10 (HAM) powdered form, prepared to instructions, 10 x 1 liter package
Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
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