75006681赛默飞离心机附件Thermo Fisher

质谱的试剂用品、实验操作、仪器和样品选择方法

(Fisher M/4056/17)、Acetonitrile(Fisher A/0626/17)、Trypsin (Promega V528)、Trypsin resolve solution(Promega V530)、Ammonium bicarbonate(Sigma A6141)、ZipTip μC-18(Millipore ZTC18M096)、TFA(GE HealthCare)、0.2ml and 0.5ml EP tube(eppendorf)、50ml EP 0.5ml EP

Preparation of High Titer Adenovirus in P11 cells

(The 7.5 ml/8 plates was figured to yield approximately 13 ml of prep to go into a 12.5 ml quickseal tube prior to CsCl banding below).   -Add 0.1 vol 5% Na deoxycholate (750 m l).  Mix well and incubate at room temperature for 30 minutes. 

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2