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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
. A 50 ml culture typically yields enough RNA for 200 gel lanes (see below), and, as noted, smaller culture volumes may be used. 2. Flat mycelial pads are easier to grind than mycelial balls. Therefore, filter cultures using a Buchner funnel
image of a whole mount from a 4 wk old virgin Protocol Spread tissue on glass slide Fix in Carnoy's fixative for 2 to 4 hours at r.t. Wash in 70 % EtOH for 15 min Change gradually to distilled water
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
0.2 micron filter Fisher Scientific SCGVT05RE Construct 40 micron cell strainers Fisher Scientific
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