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文献和实验Preparation of Broth and Plates, etc.
Combine: NaCl 4 gm (Fisher #S671-500; from Material Manag.) MgSO47H2O 1.6 gm (Sigma #M-2773) Yeast Extract 4 gm (Difco #0127-05-3) N-Z
Preparation of Broth and Plates
Preparation of Broth and Plates, etc. [Use ddH2O 17 m* or better ] Recipes: 1) LB Broth Make 16 gm of LB Broth Base (Gibco #M27800C) up to 800 ml in ddH2O. Swirl to dissolve, then add 110 µl of 10 N NaOH
(Fisher M/4056/17)、Acetonitrile(Fisher A/0626/17)、Trypsin (Promega V528)、Trypsin resolve solution(Promega V530)、Ammonium bicarbonate(Sigma A6141)、ZipTip μC-18(Millipore ZTC18M096)、TFA(GE HealthCare)、0.2ml and 0.5ml EP tube(eppendorf)、50ml EP 0.5ml EP
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