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文献和实验Southern Hybridization Protocols
hours. Put x-ray film over on both sides of the membrane under a safelight, place cassette in -20 Freezer (up to 7 days) or -70C freezer, and then develop films.Preparation of Denhardts Solution (30ml) 300mg Ficoll 400 (Fisher Bioreagents) 300mg PVP
Procedures for In Vitro Production of Bovine Embryos
with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap
10‐, 25‐, 50‐, 100‐, 250‐mL round‐bottom flasks (Kontes) 4‐mL screw‐cap glass vials Rubber septa for 14/20‐ and 24
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