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- 2025年12月31日
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文献和实验Northern Blotting方法(Howell Lab)
buffer. Pour GITC/Cell mix into (14x95 mm) polyallomer tubes with 4 ml CsCl buffer. Balance and top off tubes with more GITC buffer. Spin tubes on the SW40Ti rotor for 20 hr at 32,000 rpm (18℃). Pour off liquid and keep tubes
Sauer:RNA Purification from E. coli
). DNase I requires calcium in addition to Mg++ and works better in low salt conditions (frequently overlooked facts, Ambion''s web site has some good info). Sauer:Lysing E. coli with Lysozymes So, You have a mL or so of cells in a microfuge
Gel-elongation assay for type II fatty acid synthesis
Procedure 1) FASII gel-elongation assay was done by preincubating 0.25 - 2 µg of E. coli or S. aureus lysate with a serial dilution of inhibitors at room temperature for 20 min in 50 µl of buffer containing 100 mM sodium
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