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文献和实验-833) Temed (BioRad cat# 161-0800) SafetyCoat: Baker cat# 4017-01 10% APS, Make a 10% w/v in DEPC treated H2O. Ammonium persulfate, Sigma A7460. Keep in refrigerator, make fresh every week. 10xTBE: (890 mM) 216g Trisbase Fisher
Procedures for In Vitro Production of Bovine Embryos
Stock 2: pyruvate (ml)2.01.00.5Stock 8: gentamicin ( ml)8015050Stock 7: heparin ( ml)00250**** BSA, EFAF =essentially fatty-acid free Fraction V ** Save the 12 ml remaining after preparation of medium since it can be used for Percoll preparation
Single and Double FISH protocols for Drosophila
the pellet in 100 ul RNAse-free water (see Note #3). Check the probe by loading and running 4-5ul on a conventional agarose gel (~1%). The run-off transcript should easily be detected by ethidium bromide staining (see for ex. Hughes and Krause, 1998). Probe
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