75006485赛默飞离心机附件Thermo Fisher

Systematic Evaluation of Skeletal Mechanical Function

Microcentrifuge (Fisher Scientific accuSpin Micro 17) Ashing oven (Thermo Lindberg Blue M

小RNA的Northern BLOT

while pouring. Tap the glass to get rid off bubbles. Insert comb and slowly lower the plates down to almost horizontal (put two pipette tip boxes under gel). Pour more gel around comb, wait a few minutes and then try to position the gel horizontal. Leave

Stripping Western Blots

1) After ECL development, wash membrane once for 10min with PBST. 2) Incubate the membrane in stripping buffer (see below) in a heat-sealed plastic bag for 30min at 50oC with occasional mixing. For low-abundance antigens, the strip