76002904赛默飞离心机附件Thermo Fisher

用全血直接分选 CD3+ T 细胞

. no.: 6 - 6201 - 004），內含 : 4x Fab-TACS® Gravity column (capacity pro column: 15 ml whole blood) 1x CD3 Fab-Strep, human 1x Buffer CI (10x) 1x Biotin stock solution 1x Fab-TACS® Gravity Adapter Fab-TACS® Flow Restrictor, Pack of 5   1.1 用 ddH2O 将 10

Native Chromatin Preparation and Illumina/Solexa Library Construction

1. On the day of the experiment, obtain a leukapheresis pack from a blood bank. Dilute 109 leukocytes with 1X PBS (1:1). Pipette 15 mL of LSM into a 50-mL conical tube. Carefully layer the lymphocyte suspension over the LSM. A clear boundary

[实验步骤] 由全血直接分选 CD3+ T 细胞

® Gravity Adapter 重力柱适配器(3) Quadrow 重力柱固定架2. 样本：含抗凝血剂的正常人全血（建议使用 CPD 抗凝血剂）3. 实验步驟：(1) 准备试剂使用前，所有试剂应置于室温平衡一段时间。a) 1x Buffer CI：以 dH2O，将 10x Buffer CI 稀释成 1x 工作溶液b) Fab-Strep：以 1 ml Buffer CI 溶解 Fab-Strep 冻干粉，之后将 Fab-Strep 分成 5 管，每管 200 µl，不使用的置于 -20 °