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文献和实验more than those of our X. tropicalis lines. The male and female in this image are about the same size, but on the whole females are larger then males. Protocol for Karyotyping Xenopus tadpoles 1. Prior to collecting blood for genomic DNA preparation
SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
15 ml microcentrifuge tubes 3. Water baths preset at 37°C 4. Paper towels 实验步骤 1. Add one volume of whole blood (or bone marrow) to a nuclease-free 50 ml centrifuge tube containing 3 volume of Buffer ERL. Mix
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min
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