50073666赛默飞生物安全柜配件Thermo Fishe

A quick RNA mini-prep for Neurospora mycelial cultures

is as follows: 1. Conidia from slants (grown in 16 x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C

A quick RNA mini-prep for Neurospora mycelial cultures

x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C. A 50 ml culture typically yields enough RNA

Immunostaining of Paraffin Sections

dilutions at start) antibody or preimmune control in one or two drops to sections.  Place slides in a closed 150 mm petri dish containing wet filter paper, and place overnight at 4°C.       6) The next day, wash five times over 40