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文献和实验Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
as stage X, nearly 24 h earlier in development than most electroporation protocols. Compared to in ovo electroporation, the ex ovo method allows easier embryo orientation (the posterior half of the embryo is darker than the anterior half
Cool growth competent E . coli
Cool growth competent E . coli This protocol is very effective and simple. The fact that the cells are grown at low temperature greatly increase the OD range during which the cells will prep up at high efficiency (Inoue et
Strategies to Optimize Protein Expression in E. coli
Bao, W.J., Gao, Y.G., Chang, Y.G., Zhang, T.Y., Lin, X.J., Yan, X.Z., and Hu, H.Y. 2006. Highly efficient expression and purification system of small‐size protein domains in Escherichia coli for biochemical characterization. Protein Expr
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