A2-32C赛默飞配件（样品梳）Thermo Fisher

基于PCR技术的染色质沉淀分析

(optional; see Steps 21 and 37) Horizontal gel electrophoresis tank for agarose gels (for duplex PCR only; see Steps 40-53) Standard DNA sequencing gel apparatus with 31 x 38.5-cm glass plates, 0.4-mm spacers, and a shark's-tooth comb (for hot-stop PCR

Loss of Heterozygosity

between the plates, remove them by tapping the outside of the plates or by tipping the plates upright. Insert the straight side of the comb approximately 1 cm into the gel. If bubbles are introduced at this point, remove the comb and use the teeth of the comb

小RNA的Northern BLOT

while pouring. Tap the glass to get rid off bubbles. Insert comb and slowly lower the plates down to almost horizontal (put two pipette tip boxes under gel). Pour more gel around comb, wait a few minutes and then try to position the gel horizontal. Leave