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800308沃特曼聚碳酸酯膜NUC PC 19MM 0.05uM 100/PK Whatman
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文献和实验Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
).remove and save polylysine.wash plates 2x with 10ml sterile water.Seed cells: 1/2 X split from confluent plate (approximately 2-5 x 106 cells per 100mm plate) in PC12 culture media.Incubate overnight in 10% CO2 incubator.Cells should be 50-70% confluent
A PC-based program for evaluation of Comparative Genomic Hybridization (CGH) experiments
. The maximum image size is 1320x1035x12 bit. The 100 W mercury lamp and the diaphragms of the microscope were precisely adjusted to get a homogeneous illumination of the optical field. For each metaphase spread three gray-level images were digitized
Solutions for Tissue/Cell Digestion
. 2. Filter first through Whatman #1 filter paper, then through a Millipore 0.22 um filter. 3. Dispense into 15 ml conical disposable tubes, 5 ml each. 4. Store at -20 or -70o C. Trypsin 0.05% Materials Trypsin 1:300 (GIBCO
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