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1003-150沃特曼定性滤纸GR 3 15.0CM 100/PK Whatman
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文献和实验James Hardwick angiotensin assay protocol
5. Spot 10 microliters of each supernatant onto a 2 x 2 cm phosphocellulose paper (Whatman p81). Wash the paper in 0.425% phosphoric acid (add 5 ml 85% phosphoric acid to 1 L H2O to make 0.425% phosphoric acid.) for 3 min (15 milliliters/piece
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
. A small block made of polyvinyl chloride (2.5 x 2.5 x 1.5 cm) is attached to a flexible Teflon ribbon as shown. The block should hang ~2-3 mm above the plane of the holding block (also made of polyvinyl chloride). A piece of filter paper (i.e., Whatman 3MM
domesticus x Mus spretus] F1 DNA). In the analysis of the antibody bound (B) and unbound (U) fractions (right panel), the bands representing the maternal and paternal alleles are indicated.MATERIALS Reagents100-bp DNA step ladder (for duplex PCR
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