Bst DNA Polymerase, Large Frag

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

), where we have overcome both the size limitations and the restrictions of the cloning/subcloning procedures used in the Red/ET recombineering system. We describe the transfer of a very large DNA fragment (~55 kb) from one BAC into another unrelated BAC by the means

Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase

The Klenow fragment of DNA polymerase I is a proteolytic fragment obtained by the treatment of DNA polymerase I with subtilisin. The fragment still has the polymerase and 3′–5′ exonuclease activity, but lacks the 5′–3′ exonuclease activity

Bst-catalyzed radiolabeled DNA sequencing

  Bst DNA polymerase-catalyzed radiolabeled two-step sequencing reactions   are modified from those presented earlier   by altering the absolute amounts and the relative deoxy/dideoxynucleotide ratios in the termination mixes