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- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
6 months from date of receipt.
- 英文名:
Recombinant Human Methyl-CpG-binding domain protein 2 (MBD2), partial
- 库存:
99
- 供应商:
Biorbyt
- 规格:
20 ug/100 ug/1 mg
| 规格: | 20 ug | 产品价格: | ¥5031.0 |
|---|---|---|---|
| 规格: | 100 ug | 产品价格: | ¥9009.0 |
| 规格: | 1 mg | 产品价格: | ¥36504.0 |
描述: This Recombinant Human Methyl-CpG-binding domain protein 2 (MBD2), partial spans the amino acid sequence from region 145-411aa. Purity: Greater than 85% as determined by SDS-PAGE.
别名: (Demethylase)(DMTase)(Methyl-CpG-binding protein MBD2)
形态: Liquid or Lyophilized powder
应用说明: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
生物来源: Homo sapiens (Human)
表达区域: 145-411aa
分子量: 56.3 kDa
防腐剂: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
蛋白长度: Partial
蛋白序列: ESGKRMDCPALPPGWKKEEVIRKSGLSAGKSDVYYFSPSGKKFRSKPQLARYLGNTVDLSSFDFRTGKMMPSKLQKNKQRLRNDPLNQNKGKPDLNTTLPIRQTASIFKQPVTKVTNHPSNKVKSDPQRMNEQPRQLFWEKRLQGLSASDVTEQIIKTMELPKGLQGVGPGSNDETLLSAVASALHTSSAPITGQVSAAVEKNPAVWLNTSQPLCKAFIVTDEDIRKQEERVQQVRKKLEEALMADILSRAADTEEMDIEMDSGDEA
纯度: Greater than 85% as determined by SDS-PAGE.
来源: Yeast
标签: C-terminal hFc1-tagged
研究领域: Metabolism Research
UniProt ID: Q9UBB5
保存条件: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
备注: For research use only.


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文献和实验Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation
to methylated DNA. Methyl-CpG Immunoprecipitation (MCIp) employs an immobilized recombinant human methyl-CpG binding domain protein 2, MBD2, which binds methylated CpGs in double-stranded DNA. Elution with increasing salt concentrations allows the fractionated
清楚,因此其需要后续进一步分析确定,此外,RLGS图谱不能完全确认所缺失的片段是由于甲基化所致还是由于DNA本身缺失所致[5]且结果分析复杂,不易解释。 2.3.2 MBD(Methyl-CpG binding domain column chromatography,甲基化结合区)柱层析法 根据MBD蛋白家族和MeCP2的特性,Masahiko Shiraishi等2004年[50]提出了一种新的方法MBD柱层析法,用于筛选和发现基因组中甲基化的情况。并且比较了MBD和重亚硫酸盐基因组测序法,得出:用MBD
Introduction of human 14-3-3 proteins
with a 29-kDa protein with partial sequences identical to 14-3-3zeta.In this study,the interaction between GPIb-IX and recombinant 14-3-3zeta is reconstituted.Further,we show that the 14-3-3zeta binding site in GPIb is within a 15 residue sequence at the C
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