- 询价
- BIO-RAD
- MCA755SBV440
- 2025年12月11日
- F
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Normal human blood lymphocytes.
- 亚型：
IgG2a
- 形态：
Purified IgG conjugated to StarBright Violet 440 - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
StarBright Violet 440
- 适应物种：
Human
- 保质期：
自发货之日起 12 个月
- 目录编号：
LT27
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- 抗体名：
CD27 antibody | LT27
- 规格：
100 Tests/0.5ml
Mouse anti human CD27, clone LT27 precipitates a ~55 kDa protein under reducing conditions or a ~120 kDa protein under non-reducing conditions.
储存信息
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
应用
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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文献和实验References for CD27 antibody
- Ravoet, A.M. et al. (1989) CD25 mAb: epitopes recognised, effect on lymphocyte activation, mediation of ADCC
in Leucocyte Typing IV: White Cell Differentiation Antigens Eds Knapp, W. et al. Oxford University Press pp 408-411. - Boxall, S. et al. (2004) Abnormal cell surface antigen expression in individuals with variant CD45 splicing and histiocytosis.
Pediatr Res. 55 (3): 478-84. - Griebel, P.J. et al. (2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.
Vet Immunol Immunopathol. 119: 115-22. - Becker, P.D. et al. (2010) Generation of Human Antigen-Specific Monoclonal IgM Antibodies Using Vaccinated ‘‘Human Immune System’’ Mice
PLoS One. 5(10). pii: e13137. - van Zelm, M.C. et al. (2007) Replication history of B lymphocytes reveals homeostatic proliferation and extensive antigen-induced B cell expansion.
J Exp Med. 204: 645-55. - Steger, B. et al. (2014) CD4(+)and CD8(+)T-cell reactions against leukemia-associated- or minor-histocompatibility-antigens in AML-patients after allogeneic SCT.
Immunobiology. 219 (4): 247-60.
Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
Vybrant® DyeCycle Violet Stain
Fluor® 488 and PE channels Eliminate or correct for cell aggregates during data analysis using gating or modeling software Human and rodent stem cells efflux Vybrant® DyeCycle™ Violet stain, and this is the basis for the Side Population
Subset Analysis of Human and Mouse Mature NK Cells
Identification of natural killer (NK) cell subsets has gained attention with the recent discovery that mature mouse NK cells comprise two distinct stages. Delineation of the stages is performed using the markers CD27 and CD11b on gated NK
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