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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD159a (NKG2A)
- 形态:
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件:
避光,4-8℃
- 克隆性:
REAL283
- 标记物:
PE
- 适应物种:
human
- 保质期:
24个月
- 供应商:
Miltenyi biotec
- 宿主:
cell line
- 应用范围:
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- 浓度:
1:50
- 抗体英文名:
CD159a (NKG2A) Antibody, anti-human, REAlease®
- 抗体名:
CD159a (NKG2A) Antibody, anti-human, REAlease®
- 规格:
100 tests
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文献和实验MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞
体,实现了对抗原特异性 (CD8+ T) 细胞的检测和分离 (如,流式分析) 。此技术的特点在于,荧光或磁性微珠等标记物可以通过使用 Biotin (生物素) 而去除,保留了细胞的真实特性,得到的功能性的,label-free 的细胞可用于下游的实验研究。 1) MHC I STREPTAMERS® 技术解析 MHC I STREPTAMERS® 由两部分组成: · MHC I-Strep,即连接了 Twin-Strep-tag® 和抗原肽的 MHC I 分子。 · 荧光 (PE/APC)
. 2. Combining AbC™ anti-Rat/Hamster and ArC™ Amine Reactive Compensation Bead Kits The ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) is designed to facilitate compensation when using any of the LIVE/DEAD® fixable dead cell stains
8°C. Discard the supernatant. 5) Resuspend the cells in 900 μl Buffer 1. 6) Add 100 μl pre-washed Depletion MyOne SA Dynabeads®. 7) Incubate for 15 min at 2–8°C with gentle tilting and rotation. 8)
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