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CD14 Antibody, anti-human, PE,

REAlease®, 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-132-309
  • 2025年12月09日
  • MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
  • cell line
  • human
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德国美天旎生物技术有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原：

      CD14

    • 形态：

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件：

      避光，4-8℃

    • 克隆性：

      REAL1403

    • 标记物：

      PE

    • 适应物种：

      human

    • 保质期：

      24个月

    • 供应商：

      Miltenyi biotec

    • 宿主：

      cell line

    • 应用范围：

      MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry

    • 抗体英文名：

      CD14 Antibody, anti-human, REAlease®

    • 抗体名：

      CD14 Antibody, anti-human, REAlease®

    • 规格：

      200 µL

    Detetion of CD14+ cellsClone REAL1403 is an antibody fragment derived from the full CD14 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity. | Clone REAL1403 recognizes the human CD14 antigen. CD14 is part of the functional heteromeric LPS receptor complex comprised of at least CD14, TLR4, and MD-2. It up-regulates cell surface molecules, including adhesion molecules. CD14 is strongly expressed on most human monocytes and macrophages in peripheral blood, other bodily fluids, and in various tissues such as lymph nodes and spleen. CD14 is weakly expressed on subpopulations of human neutrophils and myeloid dendritic cells. | The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

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    图标文献和实验
    该产品被引用文献
    Goyer, S. M. and Ferrero, E. (1987) Biochemical analysis of myeloid antigen and cDNA expression of gp55 (CD14). Oxford, Oxford University Press: . | Wilson, A. D. et al. (1995) Selection of monoclonal antibodies for the identification of lymphocyte surface antigens in the New World primate Saguinus oedipus oedipus (cotton top tamarin). J. Immunol. Methods 178: 195–200. | Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J Immunol 165: 6037–6046. | Qubaja, M. et al. (2009) The detection of CD14 and CD16 in paraffin-embedded bone marrow biopsies is useful for the diagnosis of chronic myelomonocytic leukemia. Virchows Arch 454 (4): 411–419. | Neu, C. et al. (2013) CD14-dependent monocyte isolation enhances phagocytosis of listeria monocytogenes by proinflammatory, GM-CSF-derived macrophages. PLoS One 8 (6): e66898. | Paccou, J. et al. (2013) Determination and modulation of total and surface calcium-sensing receptor expression in monocytes in vivo and in vitro. PLoS One 8 (10): e74800.
    相关实验

    • T-cell dependent antigen specific in situ staining (Pe)

      -conjugated secondary Ab choice: 10- add the biotin-conjugated goat anti-rabbit (50 to 100 µl, dilution 1:200) and incubate for 30 min. 11- wash thrice in TBS-T. From this step on follow either "Development (Avidin-HRP) " or "Double staining

    • Vybrant® DyeCycle Green and Orange Stains

      Stability Vybrant® DyeCycle™ Green stain Vybrant® DyeCycle™ Orange stain 400 μL 5 mM solution in dimethyl sulfoxide (DMSO

    • MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞

      浓度为 1x107cells/ 100 μl，从中取 1 - 2x106 cells； 2． 用去离子水将 10xBuffer IS 稀释为 1x，准备 3 ml 稀释后的 Buffer IS； 3．取 1ul Strep-Jactin®PE 或 Strep-Tactin®APC，0.8μl MHC I-Strep，与 1x Buffer IS 混匀为 10ul 的终体积，冰上孵育 45 分钟； 4．将上一步孵育完的 Streptamers 复合物加入到细胞悬液中轻轻吹打混匀，冰上孵育 45 分钟

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    文献支持
    CD14 Antibody, anti-human, PE, REAlease®, 200 µL
    询价