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CD3 Antibody, anti-human, FITC

, REAlease®, 100 tests in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-112-080
  • 2025年12月09日
  • Flow cytometry
  • cell line
  • human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      CD3

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,4-8℃

    • 克隆性

      REAL104

    • 标记物

      FITC

    • 适应物种

      human

    • 保质期

      24个月

    • 供应商

      Miltenyi biotec

    • 宿主

      cell line

    • 应用范围

      Flow cytometry

    • 浓度

      1:50

    • 抗体英文名

      CD3 Antibody, anti-human, REAlease®

    • 抗体名

      CD3 Antibody, anti-human, REAlease®

    • 规格

      100 tests in 200 µL

    Identification and enumeration of CD3+ cells by flow cytometryClone REAL104 is an antibody fragment derived from the full CD3 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity. | Clone REAL104 recognizes the human CD3 antigen, a single-pass type I membrane protein also known as Leu4. CD3 is present on mature human T cells, thymocytes, a subset of NK cells, and on Purkinje cells in cerebellum. It is associated with the T cell receptor (TCR) and is responsible for its signal transduction. The CD3 antigen is a complex of five invariable chains: γ, δ, ε, ζ, and η. The epitope recognized by clone REAL104 is located on the ε-chain of the CD3 complex. | The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

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    图标文献和实验
    该产品被引用文献
    Gold, D. P. et al. (1986) Isolation of cDNA clones encoding the 20K non-glycosylated polypeptide chain of the human T-cell receptor/T3 complex. Nature 321 (6068): 431–434. | D'Acquisto, F. et al. (2011) CD3+CD4-CD8- (double negative) T cells: saviours or villains of the immune response? Biochem. Pharmacol. 82 (4): 333–340. | Birnbaum, M. E. et al. (2014) Molecular architecture of the αβ T cell receptor-CD3 complex. Proc. Natl. Acad. Sci. U.S.A. 111 (49): 17576–17581.
    相关实验
    • 用全血直接分选 CD3+ T 细胞

      × Buffer CI 稀释至 1×; 1.2 用 1 ml Buffer CI 溶解冻干粉的 Fab-Strep,用移液器反复吹打,不能涡旋!溶解后的 Fab-Strep 进行分装保存(可 200µl 每管),冻存于- 20°C,避免反复冻融; 1.3 往 200µl 溶解后的 Fab-Strep 中,加入 800µl 的 Buffer CI 。此时,Fab-Strep 溶液已准备好,可直接使用了; 1.4 加入 200µl 100 mM 的生物素至 20 ml Buffer CI 中,混匀

    • [实验步骤] 由全血直接分选 CD3+ T 细胞

      ® Gravity Adapter 重力柱适配器(3) Quadrow 重力柱固定架2. 样本:含抗凝血剂的正常人全血(建议使用 CPD 抗凝血剂)3. 实验步驟:(1) 准备试剂使用前,所有试剂应置于室温平衡一段时间。a) 1x Buffer CI:以 dH2O,将 10x Buffer CI 稀释成 1x 工作溶液b) Fab-Strep:以 1 ml Buffer CI 溶解 Fab-Strep 冻干粉,之后将 Fab-Strep 分成 5 管,每管 200 µl,不使用的置于 -20 °

    • Dynabeads® CD3CD28 CTS

      Use a 1L bag for CD3 T cell separation. Add 100 ml of air to the bag. 3)        Add 5 x 108 CD3 T cells to the 1L bag in 50-60 ml Buffer 1. 4)        Add 4.0 ml (corresponding to 1.6 x 109 Dynabeads®) of washed Dynabeads® CD3⁄CD

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    文献支持
    CD3 Antibody, anti-human, FITC, REAlease®, 100 tests in 200 µL
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