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CD48 Antibody, anti-mouse, APC

, REAfinity™, 30 µg in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-124-727
  • 2025年12月09日
  • Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
  • human cell line
  • mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      CD48

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,4-8℃

    • 克隆性

      REA1238

    • 标记物

      APC

    • 适应物种

      mouse

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry

    • 浓度

      1:50

    • 抗体英文名

      CD48 Antibody, anti-mouse, REAfinity™

    • 抗体名

      CD48 Antibody, anti-mouse, REAfinity™

    • 规格

      30 µg in 200 µL

    Identification and enumeration of CD48+ cells by flow cytometryClone REA1238 reacts with the CD48 mouse antigen, which is a 45 kDa GPI-linked glycoprotein also known as BCM1, blast-1 (human), or OX-45 (rat). CD48 belongs to the SLAM family including CD48, CD244, and CD150. These cell surface receptors were differentially expressed among functionally progenitors. Hematopoietic stem cells are highly purified as CD150–CD48–CD244– cells, multipotent hematopoietic progenitors are CD244+CD150–CD48– and most restricted progenitor cells are CD48+CD244+CD150–. In mouse, the ligands for CD48 are CD2 and CD244. | Additional information: Clone REA1238 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Latchman, Y. et al. (1998) Cutting edge: Identification of the 2B4 molecule as a counter-receptor for CD48. J Immunol 161: 5809–5812. | Kato, K. et al. (1992) CD48 is a counter-receptor for mouse CD2 and is involved in T cell activation. J. Exp. Med. 176: 1241. | Kiel, M. J. et al. (2005) SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. Cell 121 (7): 1109–1121.
    相关实验
    • APC和TSC-2 cDNA的长5'RACE

      30s,接着40个循环的94℃ 15 s,65℃ 20 s,68℃ 5 min],其中使用了1单位的ELONGASE酶混合物[PCR 50ml终体积,其中60 mM Tris-SO4(pH9.1),18 mM (NH4)2SO4,2 mM MgSO4,dNTP每个200 µM,引物每个400 nM ],其中分别是AAP和引物TSC-2-2826(A带)或引物TSC-2-1732(B带)一起使用。每个PCR产物(带1和带3)取10µl 在0.8%琼脂糖上,1倍TAE凝胶电泳和SYBRò绿染色(分子

    • AbC Anti-Mouse Bead Kit

      实验步骤   1.  Using the AbC Anti-Mouse Bead Kit 1)        Completely resuspend the AbC capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)        Label

    • ANTIBODY BINDING TO PROTEIN A AND PROTEIN G

      of various immunoglobulines. Protein A is a 42 kDa polypeptide that binds the residues in the CH2 and CH3 domains of immunoglobulin heavy chains. Protein G is an approximatley 30 kDa polypeptide that binds immunoglobulins and albumin. Recombinant protein G

    图标技术资料

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    CD48 Antibody, anti-mouse, APC, REAfinity™, 30 µg in 200 µL
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