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- 文献和实验
- 技术资料
- 免疫原:
CD49b
- 亚型:
recombinant human IgG1
- 形态:
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件:
避光,4-8℃
- 克隆性:
REA981
- 标记物:
PE-Vio 615
- 适应物种:
mouse, rat
- 保质期:
24个月
- 供应商:
Miltenyi Biotec
- 宿主:
human cell line
- 应用范围:
Flow cytometry
- 浓度:
1:50
- 抗体英文名:
CD49b Antibody, anti-mouse/rat, REAfinity™
- 抗体名:
CD49b Antibody, anti-mouse/rat, REAfinity™
- 规格:
150 µg in 1 mL
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文献和实验; 50%; PBT 30% MeOH; 70%; PBT 100% PBT For larval imaginal discs, skip the re-hydration step and start directly at the RNAseA step below, after fixation; Incubate tissues in 1ml of 100-200µg/ml RNAseA in PBT
Fluorescent in situ Hybridization Combined with Immunostaining on Polytene Chromosomes
solution 2 3.7% paraformaldehyde, 1% Triton X-100, in PBS (37% p-formaldehyde stock solution: weigh 1.85g of p-formaldehyde powder. Add in H2 O (final volume of 5ml), add 70µl 1 N KOH, dissolve by boiling). solution
with the Western blot procedure as usual. Example Antibody gives good positive results at 2 µg/mL. Normally nitrocellulose is incubated in a final volume of 10 mLs. Combine 20 µg of the antibody with 100 µg of the peptide in 1mL of P(T)BS-milk. Incubate
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