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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD206 (MMR)
- 亚型:
recombinant human IgG1
- 形态:
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件:
避光,4-8℃
- 克隆性:
REA1447
- 标记物:
PE
- 适应物种:
human, mouse
- 保质期:
24个月
- 供应商:
Miltenyi Biotec
- 宿主:
human cell line
- 应用范围:
MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
- 抗体英文名:
CD206 (MMR) Antibody, anti-human/mouse, REAfinity™
- 抗体名:
CD206 (MMR) Antibody, anti-human/mouse, REAfinity™
- 规格:
200 µL
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
× Buffer CI 稀释至 1×; 1.2 用 1 ml Buffer CI 溶解冻干粉的 Fab-Strep,用移液器反复吹打,不能涡旋!溶解后的 Fab-Strep 进行分装保存(可 200µl 每管),冻存于- 20°C,避免反复冻融; 1.3 往 200µl 溶解后的 Fab-Strep 中,加入 800µl 的 Buffer CI 。此时,Fab-Strep 溶液已准备好,可直接使用了; 1.4 加入 200µl 100 mM 的生物素至 20 ml Buffer CI 中,混匀
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