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CD107b Antibody, anti-mouse, P

E, REAfinity™, 30 µg in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-122-104
  • 2025年12月09日
  • Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
  • human cell line
  • mouse
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德国美天旎生物技术有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原：

      CD107b

    • 亚型：

      recombinant human IgG1

    • 形态：

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件：

      避光，2-8℃

    • 克隆性：

      REA1182

    • 标记物：

      PE

    • 适应物种：

      mouse

    • 保质期：

      24个月

    • 供应商：

      Miltenyi Biotec

    • 宿主：

      human cell line

    • 应用范围：

      Intracellular flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry

    • 浓度：

      1:50

    • 抗体英文名：

      CD107b Antibody, anti-mouse, REAfinity™

    • 抗体名：

      CD107b Antibody, anti-mouse, REAfinity™

    • 规格：

      30 µg in 200 µL

    Identification and enumeration of CD107b+ cells by flow cytometryClone REA1182 recognizes the mouse CD107b antigen, a highly glycosylated 110 kDa single-pass type I membrane protein also known as lysosome-associated membrane protein-2 (LAMP-2) or Mac-3 antigen. It is widely located in lysosomal membranes and the plasma membrane of macrophages and some myeloid cell lines. Under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, in malignant carcinoma cells, and in cytotoxic T lymphocytes, it is also found at the cell surface. CD107b has been described as a receptor for the selective import and degradation of cytosolic proteins in the lysosome or chaperone-mediated autophagy. | Additional information: Clone REA1182 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Cha, Y. et al. (1990) The cDNA sequence of mouse LAMP-2. Evidence for two classes of lysosomal membrane glycoproteins. J. Biol. Chem. 265 (9): 5008–5013. | Tanaka, Y. et al. (2000) Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice. Nature 406 (6798): 902–906. | Asaoka, Y. et al. (2013) Immunohistochemistry of LAMP-2 and adipophilin for phospholipidosis in liver and kidney in ketoconazole-treated mice. Exp. Toxicol. Pathol. 65 (6): 817–823.
    相关实验

    • AbC Anti-Mouse Bead Kit

      实验步骤   1.  Using the AbC™ Anti-Mouse Bead Kit 1)        Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)        Label

    • T-cell dependent antigen specific in situ staining (Pe)

      6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block

    • Immunofluorescent Staining of Mouse and Rat Leukocytes

      16 (Mouse BD Fc Block™; Cat No. 553141/553142) or anti-rat CD32 (Rat BD Fc Block™; Cat. No. 550270/550271) is recommended. BD Fc Block™ can be added to cells (~0.25 µg per million cells, 3 - 5 min, 4°C) and need not be washed out prior to addition of primary

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    CD107b Antibody, anti-mouse, PE, REAfinity™, 30 µg in 200 µL
    询价