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SCIMP Antibody, anti-human, PE

, REAfinity™, 100 tests in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-127-088
  • 2025年12月09日
  • Intracellular flow cytometry
  • human cell line
  • human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      SCIMP

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      REA1292

    • 标记物

      PE

    • 适应物种

      human

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Intracellular flow cytometry

    • 浓度

      1:50

    • 抗体英文名

      SCIMP Antibody, anti-human, REAfinity™

    • 抗体名

      SCIMP Antibody, anti-human, REAfinity™

    • 规格

      100 tests in 200 µL

    Identification and enumeration of SCIMP+ cells by flow cytometryClone REA1292 recognizes the human SCIMP, a membrane protein, which belongs to family of transmembrane adaptor proteins (TRAP). In a phosphorylated state, SCIMP binds to SLP65/SLP76 and inhibitory kinase Csk. It is expressed in antigen presenting cells (APC), including B cells, macrophages, and bone marrow derived dendritic cells. It may play a role in signal transduction after MHC-II stimulation and functions as a regulator of antigen presentation and other functions of antigen presenting cells. | Additional information: Clone REA1292 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Williamson, M. P. (1994) The structure and function of proline-rich regions in proteins. Biochem. J. 297 (2): 249–260. | Draber, P. et al. (2011) SCIMP, a transmembrane adaptor protein involved in major histocompatibility complex class II signaling. Mol. Cell. Biol. 31 (22): 4550–4562.
    相关实验
    • T-cell dependent antigen specific in situ staining (Pe)

      -conjugated secondary Ab choice: 10- add the biotin-conjugated goat anti-rabbit (50 to 100 µl, dilution 1:200) and incubate for 30 min. 11- wash thrice in TBS-T. From this step on follow either "Development (Avidin-HRP) " or "Double staining

    • AbC Anti-Mouse Bead Kit

      suspension. 5)        Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC capture beads (Component A in the AbC Anti-Mouse Bead Kit) to each tube. 6)        Add a pre-titrated

    • AbC Anti-Rat/Hamster Bead Kit

      1 μL   5)      Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC capture beads (Component A in the AbC Anti-Mouse Bead Kit) to each tube. 6)     

    图标技术资料

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    SCIMP Antibody, anti-human, PE, REAfinity™, 100 tests in 200 µL
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