IgE Antibody, anti-human, APC, REAfinity™, 30 tests in 60 µL产品图

IgE Antibody, anti-human, APC,

REAfinity™, 30 tests in 60 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-118-003
  • 2026年05月28日
  • Flow cytometry
  • human cell line
  • human, non-human primate
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  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      IgE

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      REA1049

    • 标记物

      APC

    • 适应物种

      human, non-human primate

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Flow cytometry

    • 浓度

      1:50

    • 抗体英文名

      IgE Antibody, anti-human, REAfinity™

    • 抗体名

      IgE Antibody, anti-human, REAfinity™

    • 规格

      30 tests in 60 µL

    Identification and enumeration of IgE+ cells by flow cytometryClone REA1049 recognizes the IgE isotype of human immunoglobulins. IgE is the shortest-lived immunoglobulin with a half-life of two days in serum. IgE plays an important role in allergy. It is especially associated with type 1 hypersensitivity, where IgE shows high-affinity binding to Fc receptors expressed on the surface of mast cells. Antigen binding to Fc receptor–bound IgE upon re-exposure to specific allergens results in degranulation and the release of a variety of mediators, such as histamine and cytokines. | IgE has two main receptors: the high-affinity receptor FcεRI, which is expressed only on mast cells and basophils, and the low-affinity receptor FcεRII (CD23), which is expressed on B cells. | Additional information: Clone REA1049 displays negligible binding to Fc receptors.

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    图标文献和实验
    相关实验
    • APC和TSC-2 cDNA的长5'RACE

      30s,接着40个循环的94℃ 15 s,65℃ 20 s,68℃ 5 min],其中使用了1单位的ELONGASE酶混合物[PCR 50ml终体积,其中60 mM Tris-SO4(pH9.1),18 mM (NH4)2SO4,2 mM MgSO4,dNTP每个200 µM,引物每个400 nM ],其中分别是AAP和引物TSC-2-2826(A带)或引物TSC-2-1732(B带)一起使用。每个PCR产物(带1和带3)取10µl 在0.8%琼脂糖上,1倍TAE凝胶电泳和SYBRò绿染色(分子

    • Detection of Known Allergen-Specific IgE Antibodies by Immunological Methods

      are incubated with the respective diluted serum sample (approximately 25 μL serum required) and bound IgE antibodies are detected with a secondary horseradish peroxidase (HRP) labelled anti-human-IgE antibody via chemiluminescence. The measurement

    • AbC Anti-Mouse Bead Kit

      实验步骤   1.  Using the AbC Anti-Mouse Bead Kit 1)        Completely resuspend the AbC capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)        Label

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    IgE Antibody, anti-human, APC, REAfinity™, 30 tests in 60 µL
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