- 询价
- Miltenyi Biotec
- 德国
- 130-120-433
- 2025年12月09日
- Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Mass cytometry
- human cell line
- human, non-human primate
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
HLA-ABC
- 亚型：
recombinant human IgG1
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，2-8℃
- 克隆性：
REA230
- 标记物：
PE-Vio 770
- 适应物种：
human, non-human primate
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
human cell line
- 应用范围：
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Mass cytometry
- 浓度：
1:50
- 抗体英文名：
HLA-ABC Antibody, anti-human, REAfinity™
- 抗体名：
HLA-ABC Antibody, anti-human, REAfinity™
- 规格：
100 tests in 200 µL
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文献和实验together to calculate compensation in multicolor immunophenotyping experiments that incorporate a LIVE/DEAD® fixable dye by following the protocol outlined below: 1) Gently vortex the ArC™ Amine Reactive Beads Kit and the AbC™ Anti-Mouse Bead Kit
1 μL 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6)
MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞
浓度为 1x107cells/ 100 μl，从中取 1 - 2x106 cells； 2． 用去离子水将 10xBuffer IS 稀释为 1x，准备 3 ml 稀释后的 Buffer IS； 3．取 1ul Strep-Jactin®PE 或 Strep-Tactin®APC，0.8μl MHC I-Strep，与 1x Buffer IS 混匀为 10ul 的终体积，冰上孵育 45 分钟； 4．将上一步孵育完的 Streptamers 复合物加入到细胞悬液中轻轻吹打混匀，冰上孵育 45 分钟
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