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CD88 (C5AR) Antibody, anti-hum

an, PE, 100 tests in 1 mL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-104-285
  • 2025年12月09日
  • Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
  • mouse
  • human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      CD88 (C5AR)

    • 亚型

      mouse IgG2aκ

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      S5/1

    • 标记物

      PE

    • 适应物种

      human

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      mouse

    • 应用范围

      Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry

    • 浓度

      1:11

    • 靶点

      CD88 (C5AR)

    • 抗体英文名

      CD88 (C5AR) Antibody, anti-human

    • 抗体名

      CD88 (C5AR) Antibody, anti-human

    • 规格

      100 tests in 1 mL

    Identification and enumeration of CD88 (C5AR)+ cells by flow cytometryClone S5/1 recognizes the human CD88 antigen, a 43 kDa G type protein–coupled receptor with seven membrane-spanning regions which is also known as C5a anaphylatoxin chemotactic receptor 1 (C5AR). CD88 expression is originally described on neutrophils and monocyte/macrophages, but it is also found on hepatocytes, epithelial cells, endothelial cells, tissue mast cells, and human fetal astrocytes. Constitutive CD88 expression was also detected on astrocytes, microglia, and fibroblast-like cells but not by oligodendrocytes. CD88 is a receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. It stimulates chemotaxis, granule enzyme release, and superoxide anion production. Sulfation plays a critical role in the association of CD88 with C5a, but no significant role in the ability of CD88 to transduce a signal and mobilize calcium in response to a small peptide agonist.

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    图标文献和实验
    相关实验
    • T-cell dependent antigen specific in situ staining (Pe)

      and then apply 3% human serum as a blocking agent (health hazard!). 4- incubate with the blocking for 10 min. 5- blot the slides without washing and apply Phycoerythrin (B-Pe or R-Pe) 0.5µg/ml in PBS-BSA-NaN 3 , in a moist chamber, at RT for 1-18 hr.

    • Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum

      Sample buffer, one 60‐ml bottle Wash buffer, phosphate‐saline concentrate, 5×, one 100 ml bottle: transfer contents to a graduated cylinder, bring volume up to 500 ml

    • Phycoerythrin conjugation protocol

      and Centricon concentrators. Add 0.5% sodium azide. Store at 4 °C protected from light. Do not freeze.     Figure 1. Analysis of column eluate fractions from PE conjugation of monoclonal antibody IB4 (murine IgG2a, anti-human CD

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