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Rae-1 Pan Specific Antibody, a

nti-mouse, FITC, REAfinity™, 30 µg in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-111-468
  • 2025年12月09日
  • Flow cytometry
  • human cell line
  • mouse
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德国美天旎生物技术有限公司
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    • 文献和实验
    • 技术资料
    • 免疫原：

      Rae-1 Pan Specific

    • 亚型：

      recombinant human IgG1

    • 形态：

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件：

      避光，2-8℃

    • 克隆性：

      REA723

    • 标记物：

      FITC

    • 适应物种：

      mouse

    • 保质期：

      24个月

    • 供应商：

      Miltenyi Biotec

    • 宿主：

      human cell line

    • 应用范围：

      Flow cytometry

    • 浓度：

      1:50

    • 抗体英文名：

      Rae-1 Pan Specific Antibody, anti-mouse, REAfinity™

    • 抗体名：

      Rae-1 Pan Specific Antibody, anti-mouse, REAfinity™

    • 规格：

      30 µg in 200 µL

    Identification and enumeration of Rae-1 pan specific+ cells by flow cytometryClone REA723 recognizes the Rae-1 pan specific antigen, a surface protein and member of GPI-linked (glypiated) proteins which share partial homology with MHC class I molecules. Rae-1 was detected in early mouse embryos, especially in the head region. Rae-1 is also up-regulated on a large variety of tumor cells. Together with H-60, Rae-1 is a ligand for the murine NKG2D receptor and a potent stimulator of innate immunity. The ligand-expressing tumor cells induce potent priming of cytotoxic T cells and sensitization of NK cells in vivo. Clone REA723 detects Rae-1α/β/γ/δ/ε. | Additional information: Clone REA723 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Nomura, M. et al. (1996) Genomic structures and characterization of Rae1 family members encoding GPI-anchored cell surface proteins and expressed predominantly in embryonic mouse brain. J. Biol. Chem. 120 (5): 987–995. | Diefenbach, A. et al. (2000) Ligands for the murine NKG2D receptor: expression by tumor cells and activation of NK cells and macrophages. Nat Immunol 1 (2): 119–126. | Diefenbach, A. et al. (2001) Rae1 and H60 ligands of the NKG2D receptor stimulate tumour immunity. Nature 413 (6852): 165–171.
    相关实验

    • AbC Anti-Mouse Bead Kit

      实验步骤   1.  Using the AbC™ Anti-Mouse Bead Kit 1)        Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)        Label

    • Isotype-specific immunostaining with mouse primary antibodies on mouse tissue

      ) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a

    • T-cell dependent antigen specific in situ staining (Pe)

      -conjugated secondary Ab choice: 10- add the biotin-conjugated goat anti-rabbit (50 to 100 µl, dilution 1:200) and incubate for 30 min. 11- wash thrice in TBS-T. From this step on follow either "Development (Avidin-HRP) " or "Double staining

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    文献支持
    Rae-1 Pan Specific Antibody, anti-mouse, FITC, REAfinity™, 30 µg in 200 µL
    询价