- 询价
- Miltenyi Biotec
- 德国
- 130-102-632
- 2025年12月09日
- Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Immunofluorescence, Immunohistochemistry
- hamster
- mouse, rat, non-human primate
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CD81
- 亚型：
hamster IgGκ
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，2-8℃
- 克隆性：
EAT2
- 标记物：
PE
- 适应物种：
mouse, rat, non-human primate
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
hamster
- 应用范围：
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Immunofluorescence, Immunohistochemistry
- 浓度：
1:10
- 靶点：
CD81
- 抗体英文名：
CD81 Antibody, anti-mouse/rat
- 抗体名：
CD81 Antibody, anti-mouse/rat
- 规格：
30 µg in 1 mL
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文献和实验Western Blotting with Horseradish Pe
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Phycoerythrin conjugation protocol
1 ml purified antibody at approx 2.5 mg/ml in PBS. Antibody must be fairly pure - i.e. purify on protein A or G column before conjugating. Add 20 µl SMCC, 1.7 mg/ml in DMSO. Incubate for 1 hour. Take product from step (2) and add
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