- 询价
- Miltenyi Biotec
- 德国
- 130-102-630
- 2025年12月09日
- Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Immunofluorescence, Immunohistochemistry
- hamster
- mouse, rat, non-human primate
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CD81
- 亚型：
hamster IgGκ
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，2-8℃
- 克隆性：
EAT2
- 标记物：
APC
- 适应物种：
mouse, rat, non-human primate
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
hamster
- 应用范围：
Flow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry, Immunofluorescence, Immunohistochemistry
- 浓度：
1:10
- 靶点：
CD81
- 抗体英文名：
CD81 Antibody, anti-mouse/rat
- 抗体名：
CD81 Antibody, anti-mouse/rat
- 规格：
30 µg in 1 mL
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文献和实验Coating of Platelets with Antibody in vitro
or an Ab directed to platelet Ag (you may use Grener F-bottom microtitration plate for this step). If the Ab used is rat IgG1 (k) against mouse CD41 (MWReg30, Becton Dickinson, Cat.: 09911D) then the advised concentration is 0.6µg/ml (0.15µg per 200µl
1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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