- ￥8710
- biorbyt
- orb1519933
- 英国
- 2025年12月08日
- FC, ICC, IF, IHC, IP, WB
- Rabbit
- Human
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- 详细信息
- 技术资料
- 抗体名：
Rabbit anti-LAG3 Recombinant Monoclonal Antibody抗体
- 抗体英文名：
Rabbit anti-LAG3 Recombinant Monoclonal Antibody
- 靶点：
LAG3
- 浓度：
500 µg/ml
- 应用范围：
FC, ICC, IF, IHC, IP, WB
- 宿主：
Rabbit
- 适应物种：
Human
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb1519933
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Recombinant
- 保存条件：
参考说明书
- 形态：
Whole IgG
- 免疫原：
Between 475 and C-terminus
- 规格：
100 ul
产品描述：LAG3 Antibody
别名:lymphocyte-activation gene 3; Protein FDC; lymphocyte activation gene 3 protein; LAG-3; CD223; CD antigen CD223
免疫原：Between 475 and C-terminus
克隆性:BLR027F
应用稀释比例：Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Immunoprecipitation (IP) 12 µl/mg lysate. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
应用注释：All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight
纯度:Recombinant antibody was purified from cell culture supernatant
防腐剂:Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
保存说明:2 - 8°C
NCBI:NP_002277.4
UniProt ID:P18627
Note:For research use only.
别名:lymphocyte-activation gene 3; Protein FDC; lymphocyte activation gene 3 protein; LAG-3; CD223; CD antigen CD223
免疫原：Between 475 and C-terminus
克隆性:BLR027F
应用稀释比例：Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Immunoprecipitation (IP) 12 µl/mg lysate. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
应用注释：All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight
纯度:Recombinant antibody was purified from cell culture supernatant
防腐剂:Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
保存说明:2 - 8°C
NCBI:NP_002277.4
UniProt ID:P18627
Note:For research use only.
Detection of human LAG3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-LAG3 recombinant monoclonal antibody (orb1519933) used for IP at 12 µl per reaction.
Detection of human LAG3 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (orb1519933) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).
Rabbit anti-CD3E recombinant monoclonal, Rabbit anti-CD4 recombinant monoclonal, rabbit anti-CD8 alpha recombinant monoclonal, mouse anti-cytokeratin monoclonal, rabbit anti-FOXP3 recombinant monoclonal, and rabbit anti-LAG3 recombinant monoclonal. Secondary: HRP-conjugated goat anti-rabbit IgG and HRP-conjugated goat anti-mouse IgG. Substrate: Opal™ 480, 520, 570, 620, 690 and 780. Counterstain: DAPI (blue)
Detection of human LAG3 in FFPE tonsil by immunohistochemistry. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Detection of human LAG3 in FFPE HDLM-2 by immunocytochemistry. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB
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Rabbit anti-LAG3 Recombinant Monoclonal Antibody抗体，orb1519933，biorbyt
￥8710
