PGK1 Mouse Monoclonal Antibody 抗体,orb1474128,Biorbyt产品图

PGK1 Mouse Monoclonal Antibody

抗体,orb1474128,Biorbyt
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  • ¥1638 - 4329
  • Biorbyt已认证
  • orb1474128
  • 英国
  • 2026年08月06日
  • FC, IH, KO/KD Validated, WB
  • Mouse
  • Human, Mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      PGK1 Mouse Monoclonal Antibody 抗体

    • 抗体英文名

      PGK1 Mouse Monoclonal Antibody

    • 靶点

      PGK1

    • 应用范围

      FC, IH, KO/KD Validated, WB

    • 宿主

      Mouse

    • 适应物种

      Human, Mouse

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb1474128

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Monoclonal

    • 保存条件

      详询

    • 形态

      Liquid

    • 免疫原

      详询

    • 规格

      30 ul/50 ul/100 ul/200 ul

    规格:30 ul产品价格:¥1638.0
    规格:50 ul产品价格:¥2106.0
    规格:100 ul产品价格:¥3159.0
    规格:200 ul产品价格:¥4329.0
    类别: Antibodies

    产品子类型: Primary Antibody

    描述: The PGK1 Antibody [KO/KD Validated] is suitable for FC, IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名: PGKA; Phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2; PRP 2

    应用稀释比例: WB (1/500 - 1/2000), IH (1/50 - 1/100), FC (1/50 - 1/100)

    免疫原: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.

    防腐剂: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    纯化方法: The antibody was purified by immunogen affinity chromatography.

    特异性: Recognizes endogenous levels of PGK1 protein.

    研究领域: Metabolism Research

    UniProt ID: P00558, P09411

    Entrez 基因 ID: 18655, 5230

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Western blot analysis of PGK1 expression in HEK293T (A), A431 (B), mouse brain (C) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 44 kD)

    产品细节图片2
    Immunohistochemical analysis of PGK1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Overlay histogram showing Jurkat cells stained with Anti-PGK1 Antibody (green line). The cells were fixed with 2% Fixative (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by Anti-PGK1 Antibody for 60 min at 37 °C. The secondary antibody used was Goat Anti-Mouse IgG (H&L) - AF488 at 1/200 dilution for 40 min at 37 °C. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

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    ¥1638 - 4329