- ￥3250
- biorbyt
- orb764275
- 英国
- 2025年12月05日
- ELISA, IF, IHC-P, WB
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Rb (phospho Ser780) rabbit pAb抗体
- 抗体英文名：
Rb (phospho Ser780) rabbit pAb
- 浓度：
1 mg/ml
- 应用范围：
ELISA, IF, IHC-P, WB
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb764275
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 亚型：
IgG
- 免疫原：
The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser780. AA range:751-800
- 规格：
50 ul
别名:Anti-RB1 antibody, anti-Retinoblastoma-associated protein antibody, anti-p105-Rb antibody, anti-pRb antibody, anti-Rb antibody, anti-pp110 antibody
免疫原：The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser780. AA range:751-800
分子量:106
应用稀释比例：Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
防腐剂:PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
纯化:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
来源:Rabbit
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Note:For research use only.
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文献和实验Generation and Application of Phospho-specific Antibodies for p53 and pRB
of phosphoproteins. We have improved methods for generation of phosphorylation site-specific antibodies and have successfully obtained antibodies for the analysis of most of the phosphorylation sites on p53 and RB proteins.
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
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