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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
p53 (phospho Ser33) rabbit pAb抗体
- 抗体英文名:
p53 (phospho Ser33) rabbit pAb
- 浓度:
1 mg/ml
- 应用范围:
ELISA, WB
- 适应物种:
Human, Mouse, Rat
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb764259
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 亚型:
IgG
- 免疫原:
The antiserum was produced against synthesized peptide derived from human p53 around the phosphorylation site of Ser33. AA range:1-50
- 规格:
50 ul
别名:Anti-TP53 antibody, anti-P53 antibody, anti-Cellular tumor antigen p53 antibody, anti-Antigen NY-CO-13 antibody, anti-Phosphoprotein p53 antibody, anti-Tumor suppressor p53 antibody
免疫原:The antiserum was produced against synthesized peptide derived from human p53 around the phosphorylation site of Ser33. AA range:1-50
分子量:48
应用稀释比例:Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
防腐剂:PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
纯化:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
来源:Rabbit
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Note:For research use only.


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文献和实验dongreyuan 有的抗体名称上面写着Ab-33,比如,β-catenin(Ab-33)antibody,请问,Ab-33是什么意思? bettyzyj 这个抗体是非磷酸化的抗体non-phospho-antibody ,33 代表的是基因位点 dongreyuan 谢谢了 yhwangcams bettyzyj wrote
Generation and Application of Phospho-specific Antibodies for p53 and pRB
of phosphoproteins. We have improved methods for generation of phosphorylation site-specific antibodies and have successfully obtained antibodies for the analysis of most of the phosphorylation sites on p53 and RB proteins.
、ATM、ATR和chkl/2等。这些蛋白激酶直接或间接地磷酸化P53蛋白。磷酸化多发生于P53蛋白的N末端或C末端。但因为P53蛋白的N末端与Mdn2―2结合相关,因而N末端的磷酸化,特别是Serl5、Ser20、Ser33和Thr46的磷酸化对阻止Mdm2的结合更关键。P53蛋白乙酰化(acetylation)和素模化(sumoylation)对蛋白的稳定性及活性也有调节作用。 除了DiNA损伤,细胞生长的异常也能引起P53蛋白的激活。例如促进细胞生长的myc、fas和E2F1等癌
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