BNP Antibody抗体,orb705625,biorbyt产品图

BNP Antibody抗体,orb705625,biorb

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  • ¥1560
  • biorbyt已认证
  • orb705625
  • 英国
  • 2025年12月05日
  • IF, IHC, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      BNP Antibody抗体

    • 抗体英文名:

      BNP Antibody

    • 应用范围:

      IF, IHC, WB

    • 宿主:

      Rabbit

    • 适应物种:

      Human, Mouse, Rat

    • 保质期:

      6-12个月

    • 抗原来源:

      详询

    • 目录编号:

      orb705625

    • 级别:

      科研级

    • 库存:

      88

    • 供应商:

      biorbyt

    • 标记物:

      Unconjugated

    • 克隆性:

      Polyclonal

    • 形态:

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原:

      Recombinant fusion protein of human BNP. The exact sequence is proprietary.

    • 规格:

      30 ul

    产品描述:The BNP Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human BNP. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:Natriuretic peptides B; Gamma-brain natriuretic peptide

    免疫原:Recombinant fusion protein of human BNP. The exact sequence is proprietary.

    应用稀释比例:WB: 1:500-1:2000, IF: 1:50-1:200

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:4879, 18158, 25105

    UniProt ID:P13205, P16860, P40753

    Note:For research use only.

    产品细节图片1
    Western blot analysis of BNP expression in SHSY5Y (A), mouse heart (B), rat brain (C), rat heart (D) whole cell lysates. (Predicted band size: 14 kD; Observed band size: 14 kD)

    产品细节图片2
    Immunohistochemical analysis of BNP staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of BNP staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

    产品细节图片4
    Immunofluorescent analysis of BNP staining in rat heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    图标文献和实验
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    • BNP测定概述

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