HSC70 Antibody [KO/KD Validated]抗体,orb214062,biorbyt

HSC70 Antibody [KO/KD Validate

d]抗体,orb214062,biorbyt
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  • ¥1560
  • biorbyt已认证
  • orb214062
  • 英国
  • 2025年12月05日
  • IF, WB
  • Rabbit
  • Bovine, Gallus, Human, Mouse, Rat
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    • 详细信息
    • 技术资料
    • 抗体名

      HSC70 Antibody [KO/KD Validated]抗体

    • 抗体英文名

      HSC70 Antibody [KO/KD Validated]

    • 应用范围

      IF, WB

    • 宿主

      Rabbit

    • 适应物种

      Bovine, Gallus, Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb214062

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原

      KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSC70. The exact sequence is proprietary.

    • 规格

      30 ul

    产品描述:The HSC70 Antibody [KO/KD Validated] is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSC70. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:HSC70; HSP73; HSPA10; Heat shock cognate 71 kDa protein; Heat shock 70 kDa protein 8; Lipopolysaccharide-associated protein 1; LAP-1; LPS-associated protein 1

    免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSC70. The exact sequence is proprietary.

    应用稀释比例:WB: 1-500-1-1000, IP: 1-10-1-100

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:3312, 15481, 24468

    UniProt ID:P63017, P11142, P63018

    Note:For research use only.

    HSC70 Antibody [KO/KD Validate
    Western blot analysis of HSC70 expression in HEK293T (A), Hela (B), H460 (C), mouse heart (D), mouse brain (E), rat heart (F), rat muscle (G) whole cell lysates. (Predicted band size: 70 kD; Observed band size: 70 kD)

    HSC70 Antibody [KO/KD Validate
    Immunofluorescent analysis of HSC70 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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