- ￥1820
- biorbyt
- orb214020
- 英国
- 2025年12月05日
- IF, IHC, IP, WB
- Rabbit
- Bovine, Human, Mouse, Porcine, Rat, Zebrafish
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- 详细信息
- 技术资料
- 抗体名：
GSK3 beta (Phospho-S9) Antibody抗体
- 抗体英文名：
GSK3 beta (Phospho-S9) Antibody
- 应用范围：
IF, IHC, IP, WB
- 宿主：
Rabbit
- 适应物种：
Bovine, Human, Mouse, Porcine, Rat, Zebrafish
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb214020
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
- 免疫原：
KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S9 of human GSK3 beta protein. The exact sequence is proprietary.
- 规格：
30 ul
产品描述：The GSK3 beta (Phospho-S9) Antibody is suitable for IF, IHC, IP, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S9 of human GSK3 beta protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
别名:Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
免疫原：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S9 of human GSK3 beta protein. The exact sequence is proprietary.
应用稀释比例：WB: 1-500-1-1000, IHC-P: 1-100-1-200, IP: 1-10-1-100
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:56637, 84027, 2932
UniProt ID:P49841, Q9WV60, P18266
Note:For research use only.
别名:Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
免疫原：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S9 of human GSK3 beta protein. The exact sequence is proprietary.
应用稀释比例：WB: 1-500-1-1000, IHC-P: 1-100-1-200, IP: 1-10-1-100
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:56637, 84027, 2932
UniProt ID:P49841, Q9WV60, P18266
Note:For research use only.
Western blot analysis of GSK3 beta (Phospho-S9) expression in HeLa (A), MCF7 (B), A549 (C) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 46 kD)
Immunohistochemical analysis of GSK3 beta (Phospho-S9) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of GSK3 beta (Phospho-S9) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Direct ELISA antibody dose-response curve using Anti-GSK3 beta (Phospho-S9) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
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GSK3 beta (Phospho-S9) Antibody抗体，orb214020，biorbyt
￥1820
