PLC gamma 2 (Phospho-Y1217) Rabbit Polyclonal Antibody 抗体,orb214406,Biorbyt产品图

PLC gamma 2 (Phospho-Y1217) Ra

bbit Polyclonal Antibody 抗体,orb214406,Biorbyt
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  • ¥1872 - 5148
  • Biorbyt已认证
  • orb214406
  • 英国
  • 2026年07月28日
  • IF, IHC, WB
  • Rabbit
  • Human, Monkey, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      PLC gamma 2 (Phospho-Y1217) Rabbit Polyclonal Antibody 抗体

    • 抗体英文名

      PLC gamma 2 (Phospho-Y1217) Rabbit Polyclonal Antibody

    • 靶点

      PLCG2

    • 应用范围

      IF, IHC, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Monkey, Mouse, Rat

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb214406

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      详询

    • 形态

      Liquid

    • 免疫原

      详询

    • 规格

      30 ul/50 ul/100 ul/200 ul

    规格:30 ul产品价格:¥1872.0
    规格:50 ul产品价格:¥2340.0
    规格:100 ul产品价格:¥3627.0
    规格:200 ul产品价格:¥5148.0
    类别: Antibodies

    产品子类型: Primary Antibody

    描述: The PLC gamma 2 (Phospho-Y1217) Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1217 of human PLC gamma 2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; Phosphoinositide phospholipase C-gamma-2; Phospholipase C-IV; PLC-IV; Phospholipase C-gamma-2; PLC-gamma-2

    应用稀释比例: WB: 1-500-1-1000

    免疫原: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1217 of human PLC gamma 2 protein. The exact sequence is proprietary.

    防腐剂: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    纯化方法: The antibody was purified by immunogen affinity chromatography.

    特异性: Recognizes endogenous levels of PLC gamma 2 protein only when phosphorylated at Y1217.

    研究领域: Cardiovascular Research

    UniProt ID: Q8CIH5, P16885, P24135

    Entrez 基因 ID: 29337, 5336, 234779

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Western blot analysis of PLC gamma 2 (Phospho-Y1217) expression in HCT116-EGF (A), Myla2059 (B) whole cell lysates. (Predicted band size: 147 kD; Observed band size: 150 kD)

    产品细节图片2
    Immunohistochemical analysis of PLC gamma 2 (Phospho-Y1217) staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of PLC gamma 2 (Phospho-Y1217) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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    图标文献和实验
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      cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey

    • Use of EIA to Measure MMPs and TIMPs

      the combination of a monoclonal antibody as the solid-phase with rabbit polyclonal antibody and horseradish peroxidase (HRP)-labeled donkey antirabbit gamma globulin as the second phase (2 ). EIAs for human MMP-9 were developed using MMP-9 specific polyclonal

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