- ￥7605
- Biorbyt
- orb151065
- 英国
- 2026年07月28日
- ICC, IF, IHC, WB
- Mouse
- Human, Mouse, Rat
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- 文献和实验
- 技术资料
- 抗体名：
KCNK3 Antibody (Biotin) 抗体
- 抗体英文名：
KCNK3 Antibody (Biotin)
- 靶点：
KCNK3
- 浓度：
1 mg/ml
- 应用范围：
ICC, IF, IHC, WB
- 宿主：
Mouse
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb151065
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Biotin
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
IgG2b
- 免疫原：
详询
- 规格：
100 ug
描述: Mouse monoclonal to TASK1 Potassium Channel (Biotin). K+ channels are divided into three subclasses reflecting the number of transmembrane segments (TMS), which are designated 6TMS, 4TMS and 2TMS. Members of the 4TMS class contain two distinct pore regions and include TWIK, TREK, TRAAK and TASK. TASK channels are highly sensitive to external pH in the physiological range. TASK-1 is expressed in brain and in rat heart, with high levels of expression in the right atrium. TASK-2, mainly expressed in kidney, is localized in cortical distal tubules and collecting ducts, suggesting a role in renal K+ transport. TASK-3 from rat cerebellum shares 54% identity with TASK-1, but less than 30% identity with TASK-2 and other tandem pore K+ channels..
克隆号: N374/48 (Formerly sold as S374-48)
别名: Potassium channel subfamily K member 3, KCNK3, Acid sensitive potassium channel protein TASK 1, Cardiac two pore background K(+) channel, cTBAK 1, K2p3.1, KCNK9, OAT1, rTASK, TASK 1, TBAK1, TWIK related acid sensitive K+ channel, Two pore potassium channel KT3.1, Two pore K(+) channel KT3.1
应用稀释比例: WB (1:1000), ICC/IF (1:100)
应用说明: 1 µg/ml was sufficient for detection of TASK1 Potassium Channel in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
免疫原: Fusion protein amino acids 251-411 (cytoplasmic C-terminus) of rat Acid-sensitive potassium channel protein TASK or TASK1. Mouse: 96% identity (156/161 amino acids identical). Human: 76% identity (163/161 amino acids identical). < 30% identity with TASK3.
分子量: 50kDa
防腐剂: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
纯化方法: Protein G Purified
特异性: Detects ~50kDa. Does not cross-react with TASK3.
研究领域: Neuroscience, Pharmacology & Drug Discovery
UniProt ID: O54912
NCBI 参考序列: NP_203694.1
Entrez 基因 ID: 29553
保存条件: Conjugated antibodies should be stored according to the product label
备注: For research use only.
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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