产品封面图

ATG5/APG5L Mouse Monoclonal An

tibody抗体,orb500007,biorbyt
收藏
  • ¥3120
  • biorbyt已认证
  • orb500007
  • 英国
  • 2025年12月04日
  • ICC, IF, IHC-Fr, IHC-P, WB
  • Mouse
  • Human, Mouse, Rat
avatar
品牌商
13钻石会员
  • 企业认证

    点击 QQ 联系

    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      ATG5/APG5L Mouse Monoclonal Antibody抗体

    • 抗体英文名

      ATG5/APG5L Mouse Monoclonal Antibody

    • 靶点

      ATG5

    • 浓度

      1mg/ml

    • 应用范围

      ICC, IF, IHC-Fr, IHC-P, WB

    • 宿主

      Mouse

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb500007

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Monoclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      Recombinant human ATG5 Protein full length

    • 规格

      50 ul

    产品描述:ATG5/APG5L Mouse Monoclonal Antibody

    别名:ATG5; APG 5; APG 5L; APG5; APG5 autophagy 5 like; APG5 like; APG5-like; APG5L; Apoptosis specific protein; Apoptosis-specific protein; ASP; ATG 5; ATG5; ATG5 autophagy related 5 homolog; Autophagy protein 5; hAPG5; Homolog of S Cerevisiae autophagy 5; ATG5_HUMAN.

    免疫原:Recombinant human ATG5 Protein full length

    预测反应性:Mouse

    克隆性:5D7

    分子量:32 kDa

    应用稀释比例:WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein G

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:Q9H1Y0

    Note:For research use only.

    产品细节图片1
    Hela cell, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (ATG5/APG5L) monoclonal Antibody, Unconjugated (orb500007) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

    产品细节图片2
    Hela cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (ATG5/APG5L) polyclonal Antibody, Unconjugated (orb500007) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

    产品细节图片3
    Fixative-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ATG5/APG5L) Monoclonal Antibody, Unconjugated (orb500007) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

    产品细节图片4
    Fixative-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ATG5) Monoclonal Antibody, Unconjugated (ascites of orb500007 5D7) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

    产品细节图片5
    Sample: Lane 1: Human Hela cell lysates, Lane 2: Human Panc-1 cell lysates, Lane 3: Human U87MG cell lysates, Lane 4: Human MCF-7 cell lysates, Lane 5: Human K562 cell lysates, Lane 6: Human SH-SY5Y cell lysates, Primary: Anti-ATG5/APG5L (orb500007) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 32 kDa, Observed band size: 45 kDa.

    风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    相关实验
    • Rapid Isotyping of Mouse Monoclonal Antibodies

      , isotyping is useful to control lot to lot consistency, detect contamination by another antibody, and identify hybridoma switch variants (see Notes 2 –8 for further comment on applications). Table 1  Isotype-Dependent Properties of Monoclonal

    • Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies

      Repertoires of antibody (Ab) V genes derived from nonimmunized human donors (1 ) or made synthetically (2 3 ) have been cloned for display on filamentous bacteriophage as either scFvs or Fabs fused to the minor phage coat protein (pIII

    • Monoclonal Antibody Production

      and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let

    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    ¥3120
    武汉博欧特生物科技有限公司
    2025年12月08日询价
    ¥3885
    北京智杰方远科技有限公司
    2025年05月04日询价
    ¥1500
    爱必信(上海)生物科技有限公司
    2025年05月20日询价
    询价
    南京赛戈巍生物科技有限公司
    2025年07月11日询价
    询价
    上海瑶韵生物科技有限公司
    2025年07月11日询价
    ATG5/APG5L Mouse Monoclonal Antibody抗体,orb500007,biorbyt
    ¥3120