Phospho-Cyclin B1 (Ser133) Rabbit Polyclonal Antibody 抗体,orb5927,Biorbyt产品图

Phospho-Cyclin B1 (Ser133) Rab

bit Polyclonal Antibody 抗体,orb5927,Biorbyt
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  • ¥3159 - 5850
  • Biorbyt已认证
  • orb5927
  • 英国
  • 2026年07月22日
  • FC, ICC
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Phospho-Cyclin B1 (Ser133) Rabbit Polyclonal Antibody 抗体

    • 抗体英文名

      Phospho-Cyclin B1 (Ser133) Rabbit Polyclonal Antibody

    • 靶点

      CCNB1

    • 浓度

      1mg/ml

    • 应用范围

      FC, ICC

    • 宿主

      Rabbit

    • 适应物种

      Human

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb5927

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      详询

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      详询

    • 规格

      50 ul/100 ul/200 ul

    规格:50 ul产品价格:¥3159.0
    规格:100 ul产品价格:¥4914.0
    规格:200 ul产品价格:¥5850.0
    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Phospho-Cyclin B1 (Ser133) Rabbit Polyclonal Antibody

    别名: CCNB; Ccnb1-rs1; Ccnb1-rs13; CycB1; Cycb-4; Cycb-5; Cycb1-rs1; CCNB1_HUMAN; CCNB1; CCNB1_MOUSE; Ccn-2; Cycb; CCNB1_RAT; cyclin B1; G2/mitotic-specific cyclin B1; CCNB1 | Cyclin-B1 (phospho-S133); p-Cyclin-B1; phospho-Cyclin-B1; p-CCNB1; CCNB1 | Cyclin-B1 (phospho-Ser133)

    应用稀释比例: ICC/IF=1:100-500, Flow-Cyt=1μg /Test

    免疫原: KLH conjugated Synthesised phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser133 ET(p-S)GC

    分子量: 48 kDa

    预测反应性: Mouse, Porcine, Rat

    防腐剂: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化方法: Affinity purified by Protein A

    RRID: AB_10923168

    研究领域: Signal Transduction

    UniProt ID: P14635

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Blank control (blue line): A549 (blue). Primary Antibody (green line): Rabbit Anti-Phospho-Cyclin B1 (Ser133) antibody (orb5927), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): F (ab')2 fragment goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were fixed with 2% Fixative (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Hela cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-Cyclin B1 (Ser133)) polyclonal Antibody, Unconjugated (orb5927) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

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    • 【求助】抗体名称Ab-33是什么意思?

      dongreyuan 有的抗体名称上面写着Ab-33,比如,β-catenin(Ab-33)antibody,请问,Ab-33是什么意思? bettyzyj 这个抗体是非磷酸化的抗体non-phospho-antibody ,33 代表的是基因位点 dongreyuan 谢谢了 yhwangcams bettyzyj wrote

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    ¥3159 - 5850