Stargazin Antibody (APC) 抗体,orb150200,Biorbyt产品图

Stargazin Antibody (APC) 抗体,or

b150200,Biorbyt
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  • ¥7722
  • Biorbyt已认证
  • orb150200
  • 英国
  • 2026年07月28日
  • ICC, IF, WB
  • Mouse
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      Stargazin Antibody (APC) 抗体

    • 抗体英文名:

      Stargazin Antibody (APC)

    • 靶点:

      Stargazin

    • 浓度:

      1 mg/ml

    • 应用范围:

      ICC, IF, WB

    • 宿主:

      Mouse

    • 适应物种:

      Human, Mouse, Rat

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb150200

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      APC

    • 克隆性:

      Monoclonal

    • 保存条件:

      详询

    • 亚型:

      IgG2A

    • 免疫原:

      详询

    • 规格:

      100 ug

    类别: Antibodies

    描述: Mouse monoclonal to TARP Gamma2 (APC). Stargazin belongs to a group called voltage gated calcium channels (VGCCs) which are present in most excitable cells. Stargazin is thought to stabilize the calcium channel in an inactivated (closed) state. There are five high voltage activated calcium channel types (L, N, P, Q, and R) and one low voltage activated channel type (T). Each of these channels exists as a heteromultimer of a1, b, a2/ d and g subunits with the voltage activated calcium channel function carried by the a subunits. VGCCs exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival..

    克隆号: N245/36 (Formerly sold as S245-36)

    别名: CACNG2, Calcium channel voltage dependent gamma subunit 2, CaV gamma 2, Ipr328, MGC123981, MGC138502, MGC138504, Voltage-dependent calcium channel gamma-2 subunit, Neuronal voltage-gated calcium channel gamma-2 subunit, Stargazin, Stg, Stargazer, TARP, TARP gamma-2, Transmembrane AMPAR regulatory protein gamma-2, AW060990, B230105C07Rik, B930041E13Rik, Wag, Waggler

    应用稀释比例: WB (1:1000), ICC/IF (1:100)

    应用说明: 1 µg/ml was sufficient for detection of TARP Gamma2/4/8 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    免疫原: Fusion protein amino acids 203-323 (Cytoplasmic C-terminus) of rat TARPGamma2

    分子量: 35-55kDa

    防腐剂: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA

    纯化方法: Protein G Purified

    特异性: Detects ~35-55kDa. Does not cross-react with TARPGamma3.

    研究领域: Neuroscience

    UniProt ID: Q71RJ2

    NCBI 参考序列: NP_445803.1

    Entrez 基因 ID: 84347

    保存条件: Conjugated antibodies should be stored according to the product label

    备注: For research use only.

    产品细节图片1
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody, Clone N245/36. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TARP Gamma2/4/8 (Stargazin) Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TARP Gamma2/4/8 (Stargazin) Antibody (D) Composite.

    产品细节图片2
    Western blot of rat brain lysate showing detection of ~55 kDa TARP Gamma2-4-8 protein using Anti-Tarp Gamma2-4-8 antibody [N245/36]. ?Lane 1: MW ladder. Lane 2: Anti-TARP Gamma2-4-8 (1:100). Lane 3: Anti-TARP Gamma2-4-8 (1:250). Lane 4: Anti-TARP Gamma2-4-8 (1:500). Lane 5: Anti-TARP Gamma2-4-8 (1:1000). Load: 20 μg RB lysate per lane. Block: 5% milk + TBST 1hr at RT. Primary antibody: Mouse Anti-TARP Gamma2-4-8 primary antibody [245-36] incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody at 1:50 for 60 min at RT. Colour Development: TMB solution for 30 min at RT. Predicted/Observed size: ~ 55 kDa. Other band (s) (faint): ~37kDa, ~40kDa, ~75kDa.

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