14-3-3 Alpha + Beta + Gamma +
Delta + Epsilon Rabbit Polyclonal Antibody 抗体，orb13223，Biorbyt- ￥3159 - 5850
- Biorbyt
- orb13223
- 英国
- 2026年07月22日
- FC, IF, IHC-Fr, IHC-P, WB
- Rabbit
- Human, Mouse, Rat
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- 文献和实验
- 技术资料
- 抗体名：
14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Rabbit Polyclonal Antibody 抗体
- 抗体英文名：
14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Rabbit Polyclonal Antibody
- 靶点：
YWHAB
- 浓度：
1mg/ml
- 应用范围：
FC, IF, IHC-Fr, IHC-P, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb13223
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
详询
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
详询
- 规格：
50 ul/100 ul/200 ul
| 规格： | 50 ul | 产品价格： | ￥3159.0 |
|---|---|---|---|
| 规格： | 100 ul | 产品价格： | ￥4914.0 |
| 规格： | 200 ul | 产品价格： | ￥5850.0 |
类别: Antibodies
产品子类型: Primary Antibody
描述: 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Rabbit Polyclonal Antibody
别名: GW128; HEL-S-1; HS1; KCIP-1; YWHAA; 1300003C17Rik; 14-3-3b; 1433B_BOVIN; YWHAB; Protein kinase C inhibitor protein 1 (KCIP-1); 1433B_HUMAN; Protein 1054; 1433B_MOUSE; 1433B_RAT; Prepronerve growth factor RNH-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; 14-3-3 beta; 14-3-3 alpha
应用稀释比例: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test.
免疫原: KLH conjugated synthetic peptide derived from human 14-3-3 (174-245/245aa)
分子量: 27 kDa
预测反应性: Drosophila, Sheep, Yeast
防腐剂: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化方法: Affinity purified by Protein A
RRID: AB_10752379
研究领域: Cell Biology, Cytoplasmic, Signal Transduction
UniProt ID: P31946
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
产品子类型: Primary Antibody
描述: 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Rabbit Polyclonal Antibody
别名: GW128; HEL-S-1; HS1; KCIP-1; YWHAA; 1300003C17Rik; 14-3-3b; 1433B_BOVIN; YWHAB; Protein kinase C inhibitor protein 1 (KCIP-1); 1433B_HUMAN; Protein 1054; 1433B_MOUSE; 1433B_RAT; Prepronerve growth factor RNH-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; 14-3-3 beta; 14-3-3 alpha
应用稀释比例: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test.
免疫原: KLH conjugated synthetic peptide derived from human 14-3-3 (174-245/245aa)
分子量: 27 kDa
预测反应性: Drosophila, Sheep, Yeast
防腐剂: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化方法: Affinity purified by Protein A
RRID: AB_10752379
研究领域: Cell Biology, Cytoplasmic, Signal Transduction
UniProt ID: P31946
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
Blank control (blue line): A549 (blue). Primary Antibody (green line): Rabbit Anti-14-3-3 Alpha + Beta + Gamma + Delta + Epsilon antibody (orb13223), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Blank control: A431. Primary Antibody (green line): Rabbit Anti-14-3-3 Alpha + Beta + Gamma + Delta + Epsilon antibody (orb13223), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Fixative-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (14-3-3 Alpha + Beta + Gamma + Delta + Epsilon) Polyclonal Antibody, Unconjugated (orb13223) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Fixative-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (14-3-3 Alpha + Beta + Gamma + Delta + Epsilon) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (14-3-3 Alpha + Beta + Gamma + Delta + Epsilon) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Brain Cell Lysate at 40 ug, Primary: Anti-14-3-3 Alpha + Beta + Gamma + Delta + Epsilon (orb13223) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.
Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-14-3-3 Alpha + Beta + Gamma + Delta + Epsilon (orb13223) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.
Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Uterus (Mouse) Lysate at 40 ug, Lane 3: Testis (Mouse) Lysate at 40 ug, Lane 4: Liver (Mouse) Lysate at 40 ug, Lane 5: Lung (Mouse) Lysate at 40 ug, Lane 6: Cerebrum (Rat) Lysate at 40 ug, Lane 7: Uterus (Rat) Lysate at 40 ug, Lane 8: Testis (Rat) Lysate at 40 ug, Lane 9: Liver (Rat) Lysate at 40 ug, Lane 10: Lung (Rat) Lysate at 40 ug, Lane 11: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 12: Siha (Human) Cell Lysate at 30 ug, Lane 13: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-14-3-3 Alpha+Beta+Gamma+Delta+Epsilon (orb13223) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 28 kD, Observed band size: 28 kD.
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文献和实验相关实验
, a small but significant proportion of 14-3-3 in brain is tightly and selectively associated with some membranes. Using a panel of isoform-specific antisera we find that the epsilon, eta, gamma, beta, and zeta isoforms are all present in purified synaptic
Introduction of human 14-3-3 proteins
-3-3 proteins are cytosolic,a small but significant proportion of 14-3-3 in brain is tightly and selectively associated with some membranes.Using a panel of isoform-specific antisera we find that the epsilon,eta,gamma,beta,and zeta isoforms are all present
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14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Rabbit Polyclonal Antibody 抗体，orb13223，Biorbyt
￥3159 - 5850
