- ￥3159 - 5850
- Biorbyt
- orb10183
- 英国
- 2026年07月22日
- FC, IF, IHC-Fr, IHC-P, WB
- Rabbit
- Human, Mouse, Rat
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- 文献和实验
- 技术资料
- 抗体名：
EpCAM Rabbit Polyclonal Antibody 抗体
- 抗体英文名：
EpCAM Rabbit Polyclonal Antibody
- 靶点：
EPCAM
- 浓度：
1mg/ml
- 应用范围：
FC, IF, IHC-Fr, IHC-P, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb10183
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
详询
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
详询
- 规格：
50 ul/100 ul/200 ul
| 规格： | 50 ul | 产品价格： | ￥3159.0 |
|---|---|---|---|
| 规格： | 100 ul | 产品价格： | ￥4914.0 |
| 规格： | 200 ul | 产品价格： | ￥5850.0 |
类别: Antibodies
产品子类型: Primary Antibody
描述: EpCAM Rabbit Polyclonal Antibody
别名: Ber-Ep4; BerEp4; DIAR5; EGP-2; EGP314; EGP40; ESA; HNPCC8; KS1/4; KSA; LYNCH8; M4S1; MIC18; MK-1; MOC-31; TACSTD1; TROP1; CD326; EGP; Ep-CAM; EpCAM1; GA733-2; Ly74; Tacsd1; gp40; EPCAM_HUMAN; EPCAM; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein (EGP); Epithelial glycoprotein 314 (EGP314 | hEGP314); KS 1/4 antigen; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; M1S2; EPCAM_MOUSE; Epithelial glycoprotein 314 (EGP314 | mEGP314); Protein 289A; EPCAM_RAT; Epithelial glycoprotein 314 (EGP314); Protein D5.7A; epithelial cell adhesion molecule; antigen identified by monoclonal antibody AUA1; trophoblast cell surface antigen 1
应用稀释比例: WB=1:500-2000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000, Flow-Cyt=1:100-200
免疫原: KLH conjugated synthetic peptide derived from human EpCAM (221-314/314aa)
分子量: 35-40 kDa
预测反应性: Porcine
防腐剂: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化方法: Affinity purified by Protein A
RRID: AB_10752314
研究领域: Immunology & Inflammation
UniProt ID: P16422
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
产品子类型: Primary Antibody
描述: EpCAM Rabbit Polyclonal Antibody
别名: Ber-Ep4; BerEp4; DIAR5; EGP-2; EGP314; EGP40; ESA; HNPCC8; KS1/4; KSA; LYNCH8; M4S1; MIC18; MK-1; MOC-31; TACSTD1; TROP1; CD326; EGP; Ep-CAM; EpCAM1; GA733-2; Ly74; Tacsd1; gp40; EPCAM_HUMAN; EPCAM; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein (EGP); Epithelial glycoprotein 314 (EGP314 | hEGP314); KS 1/4 antigen; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; M1S2; EPCAM_MOUSE; Epithelial glycoprotein 314 (EGP314 | mEGP314); Protein 289A; EPCAM_RAT; Epithelial glycoprotein 314 (EGP314); Protein D5.7A; epithelial cell adhesion molecule; antigen identified by monoclonal antibody AUA1; trophoblast cell surface antigen 1
应用稀释比例: WB=1:500-2000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000, Flow-Cyt=1:100-200
免疫原: KLH conjugated synthetic peptide derived from human EpCAM (221-314/314aa)
分子量: 35-40 kDa
预测反应性: Porcine
防腐剂: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化方法: Affinity purified by Protein A
RRID: AB_10752314
研究领域: Immunology & Inflammation
UniProt ID: P16422
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
Blank control: FHC. Primary Antibody (green line): Rabbit Anti-EpCAM antibody (orb10183), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Blank control: FHC. Primary Antibody (green line): Rabbit Anti-EpCAM antibody (orb10183), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Blank control: mouse kidney cells (blue). Primary Antibody: Rabbit Anti-EpCAM antibody (orb10183), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% Fixative (10 min). Primary antibody (orb10183, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.
Blank control: K562. Primary Antibody (green line): Rabbit Anti-EpCAM antibody (orb10183), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 µg/test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Blank control: K562. Primary Antibody (green line): Rabbit Anti-EpCAM antibody (orb10183), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 µg/test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Fixative-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (orb10183) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (orb10183) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (Human duodenum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (orb10183) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (Human stomach cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (orb10183) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (mouse prostate), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (orb10183) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (mouse prostate), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (orb10183) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (mouse uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (orb10183) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (orb10183) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (orb10183) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (orb10183) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (orb10183) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (orb10183) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (orb10183) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EpCAM) Polyclonal Antibody, Unconjugated (orb10183) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: MCF-7 (Human) Cell Lysate at 30 ug, Lane 3: HepG2 (Human) Cell Lysate at 30 ug, Lane 4: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-EpCAM (orb10183) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35 kD, Observed band size: 38 kD.
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EpCAM Rabbit Polyclonal Antibody 抗体，orb10183，Biorbyt
￥3159 - 5850
