•O₂⁻试剂盒，用于样本中超氧阴离子含量检测，•O₂⁻ As

Micro Superoxide Anion Assay Kit（货号：KTB1210-E）

一款基于比色法的高灵敏度检测试剂盒，用于精确定量多种生物样本中的超氧阴离子（•O₂⁻）含量，服务于细胞代谢研究与氧化应激分析领域。

产品概述

本试剂盒设计用于检测植物组织、动物组织、血清及细胞等样本中的超氧阴离子含量。超氧阴离子作为活性氧（ROS）的重要成员，在细胞信号传导与免疫反应中扮演关键角色，但其过量积累会引起氧化损伤，与多种代谢性疾病的发生发展密切相关。

检测原理

通过特异性反应体系，使样本中的•O₂⁻与试剂发生显色反应，在特定波长下测定吸光度值，依据标准曲线计算超氧阴离子含量。该方法灵敏度高，特异性良好。

组分列表

• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III
• NaNO₂ Standard (10 mmoL/L)

主要特点与优势

• 适用样本类型广泛，涵盖细胞、组织及血清等；
• 提供NaNO₂标准品及标准曲线制备方案，便于结果计算与系统校准；
• 检测灵敏度达0.001 µmoL/mL，检测范围为0.001–0.2 µmoL/mL；
• 配套详细操作指南，含样本前处理、测定步骤及结果计算说明。

注意事项

• 严禁混用不同批号或厂家的组分；
• 操作时需避免产生气泡，防止交叉污染，勤换吸头；
• 实验前需确保所有组分及设备达到适宜温度；
• 需自备CHCl3；
• 每样本需设对照孔以排除内源NO₂⁻干扰，故96孔板仅适用于48个样本检测。

保存与运输

试剂盒需避光保存于4℃。按指定条件保存，有效期为6个月（自发货日起）。运输全程采用蓝冰冰袋保障稳定性。

规格与定价

规格	价格（元）	货期
48 T/48 S	189	现货，次日发货
96 T/96 S	298	现货，次日发货

警告：本产品仅限研究使用，不适用于人类或临床诊断。使用本品前请确认相关专利与许可限制，制造商不对专利侵权或违规行为承担责任。

别名：超氧阴离子含量检测、超氧阴离子、Superoxide Anion

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Micro Superoxide Anion Assay Kit (Cat. No.: KTB1210-E)

A high-sensitivity colorimetric assay kit for the precise quantification of superoxide anion (•O₂⁻) levels in various biological samples, serving research in cellular metabolism and oxidative stress analysis.

Product Overview

This kit is designed to detect superoxide anion levels in samples such as plant tissues, animal tissues, serum, and cells. As a key member of reactive oxygen species (ROS), superoxide anion plays a critical role in cellular signaling and immune responses. However, its excessive accumulation can lead to oxidative damage and is closely associated with the pathogenesis of various metabolic diseases.

Assay Principle

Through a specific reaction system, •O₂⁻ in the sample reacts with the reagent to produce a colorimetric change. The absorbance is measured at a specific wavelength, and the superoxide anion content is calculated based on a standard curve. This method offers high sensitivity and excellent specificity.

Components List

• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III
• NaNO₂ Standard (10 mmoL/L)

Key Features and Advantages

• Broad sample compatibility, including cells, tissues, and serum;
• Provides NaNO₂ standard and standard curve preparation protocol for convenient result calculation and system calibration;
• Detection sensitivity up to 0.001 µmoL/mL, with a detection range of 0.001–0.2 µmoL/mL;
• Includes detailed operating instructions covering sample pretreatment, assay procedures, and result calculation.

Precautions

• Do not mix components from different lot numbers or manufacturers;
• Avoid bubble formation during operation to prevent cross-contamination; change pipette tips frequently;
• Ensure all components and equipment reach the appropriate temperature before starting the experiment;
• Chloroform must be self-provided;
• Each sample requires a control well to exclude interference from endogenous NO₂⁻; therefore, a 96-well plate is suitable for 48 samples only.

Storage and Shipping

The kit must be stored protected from light at 4℃. When stored under specified conditions, the shelf life is 6 months (from the date of shipment). Shipping is conducted with blue ice packs to ensure stability.

Specifications and Pricing

Specification	Price (¥)	Lead Time
48 T/48 S	189	In stock, ships next day
96 T/96 S	298	In stock, ships next day

Warning: This product is for research use only. Not intended for human or clinical diagnostic use. Verify relevant patents and licensing restrictions before using this product. The manufacturer assumes no liability for patent infringement or non-compliant use.

Alias: Superoxide Anion Content Assay, Superoxide Anion, Superoxide Anion

Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult Yakeyin Company.