- ￥189 - 298
- 亚科因(Abbkine)
- 湖北武汉
- KTB1210-S
- 2025年12月03日
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- 保存条件：
收到货后，请避光保存于4℃。请参阅说明书中各个组件的存储条件，自发货之日起，按照推荐的保存条件，有效期为6个月。
- 保质期：
收到货后，请避光保存于4℃。请参阅说明书中各个组件的存储条件，自发货之日起，按照推荐的保存条件，有效期为6个月。
- 英文名：
Micro Superoxide Anion Assay Kit
- 库存：
999
- 供应商：
亚科因(Abbkine)
- 规格：
48 T/48 S/96 T/96 S
| 规格： | 48 T/48 S | 产品价格： | ￥189.0 |
|---|---|---|---|
| 规格： | 96 T/96 S | 产品价格： | ￥298.0 |
超氧阴离子(•O₂⁻)检测试剂盒（微量法）
货号：KTB1210-S
产品分类：核心产品 - 细胞分析
产品概述
超氧阴离子试剂盒（Micro Superoxide Anion Assay Kit）是一种基于比色法的高灵敏度检测工具，用于定量测定多种生物样本中•O₂⁻的含量。该试剂盒适用于植物组织、动物组织、血清及细胞等样本，为氧化应激研究提供关键数据支持。
背景信息
超氧阴离子（•O₂⁻）是生物体内重要的活性氧之一，在免疫反应和信号传导中具有重要作用。然而，其过量积累会引起氧化应激，导致细胞膜损伤、生物大分子变性及代谢异常，与多种疾病的发生密切相关。
检测原理
本试剂盒采用经典的比色分析方法，通过特定试剂与•O₂⁻发生显色反应，在适当波长下测定吸光度值，进而计算样本中•O₂⁻的浓度。试剂盒提供NaNO₂标准品，可绘制标准曲线进行定量分析。
试剂盒组成
- Extraction Buffer
- Reagent I
- Reagent II
- Reagent III
- NaNO₂ Standard (10 mmoL/L)
特点与优势
- 广泛适用于多种生物样本类型
- 提供详细样本制备流程与结果计算指南
- 包含标准品与标准曲线参考，提高检测准确性
- 高灵敏度：最低检测浓度可达0.001 µmoL/mL
- 检测范围：0.001 - 0.2 µmoL/mL
技术参数
检测方法：比色法
检测指标：Superoxide Anion (•O₂⁻)
灵敏度：0.001 µmoL/mL
检测范围：0.001 - 0.2 µmoL/mL
规格与货期
| 规格 | 价格（元） | 货期 |
|---|---|---|
| 48 T/48 S | 189 | 现货（次日发货） |
| 96 T/96 S | 298 | 现货（次日发货） |
注意事项
- 请勿混用不同批次或不同厂家的试剂组分
- 混合或复溶试剂时应避免产生气泡
- 实验过程中应勤换吸头，防止交叉污染
- 操作前请确保所有试剂及设备处于合适温度
- 需自备CHCl3
- 96T规格可检测48个样本（每个样本需设对照孔）
保存与运输
试剂盒应在4℃避光条件下保存。请参照说明书详细了解各组分的存储要求。自发货之日起，在建议保存条件下有效期为6个月。本产品采用冰袋（蓝冰）运输。
警告
本品仅供研究使用，不适用于人类或临床诊断。使用本产品应遵守相关法律法规，不得用于专利或许可证禁止的用途。对因使用本产品可能造成的专利侵权或其他违规行为，本公司不承担任何责任。
别名：超氧阴离子含量检测, 超氧阴离子, Superoxide Anion
注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。
Superoxide Anion (•O₂⁻) Assay Kit (Micro Method)
Catalog Number: KTB1210-S
Product Category: Core Products - Cell Analysis
Product Overview
The Superoxide Anion Assay Kit (Micro Method) is a highly sensitive colorimetric-based detection tool designed for the quantitative measurement of •O₂⁻ levels in various biological samples. This kit is suitable for samples such as plant tissues, animal tissues, serum, and cells, providing critical data support for oxidative stress research.
Background Information
Superoxide anion (•O₂⁻) is one of the most important reactive oxygen species in living organisms, playing significant roles in immune responses and signal transduction. However, its excessive accumulation can induce oxidative stress, leading to cellular membrane damage, denaturation of biomacromolecules, and metabolic disorders, which are closely associated with the pathogenesis of various diseases.
Assay Principle
This kit utilizes a classic colorimetric analysis method. A specific reagent reacts with •O₂⁻ to produce a colorimetric signal, and the absorbance is measured at an appropriate wavelength to calculate the concentration of •O₂⁻ in the sample. The kit includes a NaNO₂ standard for generating a standard curve to facilitate quantitative analysis.
Kit Components
- Extraction Buffer
- Reagent I
- Reagent II
- Reagent III
- NaNO₂ Standard (10 mmoL/L)
Features and Advantages
- Broad applicability to various types of biological samples
- Provides detailed sample preparation procedures and result calculation guidelines
- Includes standards and reference standard curves to enhance detection accuracy
- High sensitivity: minimum detectable concentration as low as 0.001 µmoL/mL
- Detection range: 0.001 - 0.2 µmoL/mL
Technical Parameters
Assay Method: Colorimetric
Target Analyte: Superoxide Anion (•O₂⁻)
Sensitivity: 0.001 µmoL/mL
Detection Range: 0.001 - 0.2 µmoL/mL
Specifications and Availability
| Specification | Price (¥) | Availability |
|---|---|---|
| 48 T/48 S | 189 | In Stock (Ships Next Day) |
| 96 T/96 S | 298 | In Stock (Ships Next Day) |
Precautions
- Do not mix components from different batches or manufacturers
- Avoid generating bubbles when mixing or reconstituting reagents
- Change pipette tips frequently during experiments to prevent cross-contamination
- Ensure all reagents and equipment are at appropriate temperatures before operation
- Chloroform must be supplied by the user
- The 96T specification allows for the detection of 48 samples (each sample requires a control well)
Storage and Shipping
The kit should be stored protected from light at 4°C. Refer to the instructions for detailed storage requirements of individual components. The product is stable for 6 months from the date of shipment when stored under recommended conditions. This product is shipped with ice packs (blue ice).
Warning
This product is for research use only and is not intended for human or clinical diagnostic use. Use of this product must comply with relevant laws and regulations and must not be used for purposes prohibited by patent or license. The company assumes no liability for patent infringement or other violations that may result from the use of this product.
Also Known As: Superoxide Anion Content Assay, Superoxide Anion, Superoxide Anion
Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult Yakeyin Company.
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文献和实验1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
相关专题 在蛋白质定量试验中，最常用的方法是BCA法蛋白定量 。那么为什么我们要选择用BCA法?而且常常使用到BCA蛋白定量试剂盒（BCA Protein Assay Kit）。因为BCA法蛋白定量具有很多优点，如1.实验操作十分简单方便;2.实验耗时短，快速;3.试剂灵敏度高，实验结果准确;4.所用的试剂稳定性好，而且经济实用，抗干扰能力强等。 实验原理： BCA(bicinchoninic acid)法蛋白浓度定量
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