NADPH试剂盒，用于样本中辅酶Ⅱ含量检测，NADP(H)

NADPH试剂盒（Micro Coenzyme Ⅱ NADP(H) Assay Kit）

货号：KTB1010-E
产品分类：核心产品 - 细胞代谢检测
样本类型：适用于细胞提取物、组织样本及细菌样本

产品概述

本试剂盒是一种基于比色法的NADP/NADPH浓度检测工具，用于精确测量样本中氧化型与还原型辅酶Ⅱ（NADP⁺ 和 NADPH）的含量及其比例，适用于细胞能量代谢与氧化还原状态相关研究。

主要特点与优势

• 可分别或同时检测 NADP⁺ 和 NADPH 浓度，并计算其比值
• 线性检测范围广泛：0.5–20 µM，适用于多种生物样本
• 高兼容性：适用于细胞、组织提取物及细菌样本
• 提供完整提取缓冲液与标准品，便于样本前处理与定量校准

试剂盒组分

• Assay Buffer
• Glucose (1 M)
• WST-8 Solution
• Enhancer Solution
• NADP Cycling Enzyme Mix
• NADPH Standard (10 mM)
• NADP Extraction Buffer
• NADPH Extraction Buffer

检测参数

检测方法：比色法
检测目标：NADP/NADPH 浓度及比值
检测范围：0.5–20 µM

注意事项

为保证检测准确性，请避免样本中含有以下干扰物质：

• EDTA（＞0.5 mM）
• 抗坏血酸
• SDS（＞0.2%）
• NAN3
• NP-40（＞1%）
• 吐温-20（＞1%）

操作时请快速加入工作试剂并充分混合，以确保反应一致性。

保存与运输

保存条件：-20°C 避光保存，有效期6个月
运输方式：蓝冰低温运输

规格与价格

规格	价格（元）	货期
48 T/48 S	948	现货，次日发货
96 T/96 S	1578	现货，次日发货

应用背景

NADP/NADPH是细胞内关键辅酶，广泛参与氧化还原反应、脂质与核酸合成等生物过程。其比值可反映细胞能量状态与氧化还原平衡，是代谢研究中的重要指标。

警告：本产品仅用于科学研究，不适用于临床诊断或人体使用。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Micro Coenzyme Ⅱ NADP(H) Assay Kit

Catalog Number: KTB1010-E
Product Category: Core Products - Cell Metabolism Assays
Sample Types: Suitable for cell lysates, tissue samples, and bacterial samples

Product Overview

This kit provides a colorimetric-based method for the precise quantification of oxidized and reduced nicotinamide adenine dinucleotide phosphate (NADP⁺ and NADPH) concentrations and their ratio in samples. It is applicable to research related to cellular energy metabolism and redox status.

Key Features & Advantages

• Enables separate or simultaneous detection of NADP⁺ and NADPH concentrations, and calculation of their ratio
• Wide linear detection range: 0.5–20 µM, suitable for various biological samples
• High compatibility: applicable to cell, tissue lysates, and bacterial samples
• Includes complete extraction buffers and standards for convenient sample processing and quantitative calibration

Kit Components

• Assay Buffer
• Glucose (1 M)
• WST-8 Solution
• Enhancer Solution
• NADP Cycling Enzyme Mix
• NADPH Standard (10 mM)
• NADP Extraction Buffer
• NADPH Extraction Buffer

Assay Parameters

Assay Method: Colorimetry
Targets: NADP/NADPH concentration and ratio
Detection Range: 0.5–20 µM

Precautions

To ensure assay accuracy, avoid the presence of the following interfering substances in samples:

• EDTA (＞0.5 mM)
• Ascorbic acid
• SDS (＞0.2%)
• Sodium azide
• NP-40 (＞1%)
• Tween-20 (＞1%)

Add working reagents quickly and mix thoroughly during operation to ensure reaction consistency.

Storage & Shipping

Storage Conditions: Store at -20°C protected from light; stable for 6 months
Shipping Method: Shipped on blue ice

Specifications & Pricing

Specification	Price (¥)	Lead Time
48 T/48 S	948	In stock, ships next day
96 T/96 S	1578	In stock, ships next day

Application Background

NADP/NADPH are key coenzymes in cells, extensively involved in redox reactions, lipid and nucleic acid synthesis, and other biological processes. Their ratio reflects cellular energy status and redox balance, serving as an important indicator in metabolic research.

Warning: This product is for research use only. Not intended for clinical diagnosis or human use.

Note: The above information is subject to change due to product optimization and upgrades. Please consult Yakeyin Company for specific details.