羟甲基戊二酰辅酶A合成酶活性检测试剂盒，用于对样本中HMGC

羟甲基戊二酰辅酶A合成酶（HMGCS）活性检测试剂盒

货号：KTB3028-C

CheKine™ 羟甲基戊二酰辅酶A合成酶（HMGCS）活性检测试剂盒（微量法）是一款专业用于定量检测多种生物样本中HMGCS活性的核心试剂盒。HMGCS是甲羟戊酸途径中的关键酶，催化乙酰CoA与乙酰乙酰CoA反应生成羟甲基戊二酰CoA（HMG-CoA），该步骤是胆固醇合成及类异`戊`二`烯生物合成过程中的重要环节。

产品特点

• 经过验证的广泛样本类型支持，适用于动植物组织、细胞、细菌、真菌、血清（浆）及其他液体样本
• 操作流程简便，反应稳定，重现性良好
• 采用微量法设计，节省样本和试剂，适合高通量筛选需求

试剂盒组分

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ

检测原理

本试剂盒基于酶促反应过程中产生的CoASH可使显色剂DTNB转化为黄色TNB，通过在412 nm波长下测定吸光度变化，从而精确计算HMGCS的酶活性。

适用样本

动植物组织、细胞提取物、细菌或真菌裂解液、血清（浆）及其他生物液体样本。

保存与运输

试剂盒需-20℃避光保存，有效期为6个月。运输全程采用蓝冰冰袋低温保护，确保试剂稳定性。

注意事项

• 推荐使用新鲜样本。如不能立即检测，样本可于-80℃保存数周，避免反复冻融。
• 解冻过程需严格控制温度与时间，室温解冻应在4小时内完成。
• 实验前请确认所有试剂及设备处于适宜温度，以保证检测结果的准确性。

规格与价格

• 规格1：48 T/48 S — 价格：4716元 — 货期：现货（次日发货）
• 规格2：96 T/96 S — 价格：6126元 — 货期：现货（次日发货）

警告：本产品仅限科学研究使用，不适用于人类或临床诊断。使用者应自行负责确保应用方式不违反相关专利或许可协议。

别名：羟甲基戊二酰辅酶A合成酶（HMGCS）活性检测试剂盒，HMGCS Activity Assay Kit

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Hydroxymethylglutaryl-CoA Synthase (HMGCS) Activity Assay Kit

Catalog Number:KTB3028-C

CheKine™ Hydroxymethylglutaryl-CoA Synthase (HMGCS) Activity Assay Kit (Micro Method) is a professional core kit specifically designed for the quantitative detection of HMGCS activity in various biological samples. HMGCS is a key enzyme in the mevalonate pathway, catalyzing the condensation of acetyl-CoA and acetoacetyl-CoA to form hydroxymethylglutaryl-CoA (HMG-CoA). This step is a critical part of cholesterol synthesis and isoprenoid biosynthesis.

Features

• Validated for a wide range of sample types, including animal and plant tissues, cells, bacteria, fungi, serum (plasma), and other liquid samples
• Simple operation procedure, stable reaction, and excellent reproducibility
• Designed with a micro method, saving samples and reagents, suitable for high-throughput screening needs

Kit Components

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ

Assay Principle

This kit is based on the principle that the CoASH produced during the enzymatic reaction reduces the chromogenic agent DTNB to yellow TNB. The change in absorbance at 412 nm is measured to precisely calculate HMGCS enzyme activity.

Applicable Samples

Animal and plant tissues, cell extracts, bacterial or fungal lysates, serum (plasma), and other biological liquid samples.

Storage and Shipping

The kit must be stored protected from light at -20°C and is stable for 6 months. It is shipped with blue ice packs to ensure low-temperature protection and reagent stability throughout transit.

Precautions

• Fresh samples are recommended. If not tested immediately, samples can be stored at -80°C for several weeks; avoid repeated freeze-thaw cycles.
• The thawing process must be strictly controlled for temperature and time; thawing at room temperature should be completed within 4 hours.
• Before the experiment, ensure all reagents and equipment are at suitable temperatures to guarantee the accuracy of the detection results.

Specifications and Pricing

• Specification 1: 48 T/48 S — Price: 4716 CNY — Availability: In stock (shipped next day)
• Specification 2: 96 T/96 S — Price: 6126 CNY — Availability: In stock (shipped next day)

Warning: This product is for research use only. It is not intended for use in humans or for clinical diagnosis. The user is responsible for ensuring that the application does not infringe relevant patents or licensing agreements.

Alias: Hydroxymethylglutaryl-CoA Synthase (HMGCS) Activity Assay Kit, HMGCS Activity Assay Kit

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult YaKeYin Company.